Whole-genome amplified DNA from stored dried blood spots is reliable in high resolution melting curve and sequencing analysis 英文论文文献.docVIP
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Whole-genome amplified DNA from stored dried blood spots is reliable in high resolution melting curve and sequencing analysis 英文论文文献
Winkeletal.BMCMedicalGenetics2011,12:22
/1471-2350/12/22
TECHNICAL ADVANCE
OpenAccess
Whole-genomeamplifiedDNAfromstoreddried
bloodspotsisreliableinhighresolutionmelting
curveandsequencinganalysis
BoGWinkel1*,MadsVHollegaard2,MortenSOlesen1,JesperHSvendsen1,3,StigHauns?1,3,DavidMHougaard2,
JacobTfelt-Hansen1
Abstract
Background:Theuseofdriedbloodspots(DBS)samplesingenomicworkuphasbeenlimitedbytherelativelow
amountsofgenomicDNA(gDNA)theycontain.ItremainstobeproventhatwholegenomeamplifiedDNA
(wgaDNA)fromstoredDBSsamples,constitutesareliablealternativetogDNA.
Wewantedtocomparemeltingcurvesands
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