toxicological assessment of combined lead and cadmium_ acute and sub-chronic toxicity study in rats.pdfVIP

Food and Chemical Toxicology 65 (2014) 260–268 Contents lists available at ScienceDirect Food and Chemical Toxicology journal homepage: www.elsevi /locate/foodchemtox Toxicological assessment of combined lead and cadmium: Acute and sub-chronic toxicity study in rats Guiping Yuan a,1, Shujun Dai a,b,1, Zhongqiong Yin b,⇑,1, Hongke Lu b, Renyong Jia b, Jiao Xu b, Xu Song b, Li Li b, Yang Shu b, Xinghong Zhao b a Analytical Testing Center, Sichuan University, Chengdu 610065, China b College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China a r t i c l e i n f o a b s t r a c t Article history: The exposure to chemical mixtures is a common and important determinant of toxicity and receives Received 19 September 2013 concern for their introduction by inhalation and ingestion. However, few in vivo mixture studies have Accepted 13 December 2013 been conducted to understand the health effects of chemical mixtures compared with single chemicals. Available online 3 January 2014 In this study, the acute and 90 day sub-chronic toxicity tests of combined Pb and Cd were conducted. In the acute toxicity test, the LD50 value of Pb(NO3 )2 and CdCl2 mixture by the oral route was 2696.54 mg/kg Keywords: by Bliss method. The sub-chronic treatment revealed that the low-dose combination of Pb and Cd Pb and Cd exposures can significantly change the phys