山羊腺垂体细胞的分离培养与鉴定.pdfVIP

DOI :10.13207/ki .jnwafu.2010.01.029 38 　 1 () Vol.38 No.1 20 10 1 Journal of Northwest A F University(Nat.Sci.Ed.) Jan.2010 ＊ 尹慧勇,赵晓娥,马保华 (, , 712100) [　] 　【】, 。【】 , 、、、, , 。【】37 ℃ , 1 /L Ⅳ1 /L 2.5～ 3 h, ; 24 h ,72 h , , 6 d 。 , FS H , P RL ;, 、 。【】, , 。 [] 　;;; + [] 　Q813.1 1 [] 　A [] 　167 1-9387(20 10)01-00 11-06 Isolat ion,cult ure and ident if icat ion of goat anterior pituitary cells YIN H ui-yon ,ZHAO Xiao-e, MA Bao-hua (Key Laboratory of Animal Reproductive Physiology and Embryo Biotechnology ,Ministry of Agriculture, Collegeof Veterinary Medicine, North est A F University ,Yangling, Shaanxi 712100, China) Abstract :【Objective】T his paper focuses on the establishment of a method of the oat anterior pituita- ry cells in vitro.It can set a basis for the study of secretion characteristics invitro.【M ethod】T he effects of different enzymes,includin trypsin, colla enase Ⅳ, trypsin plus hyaluronidase, colla enase Ⅳplus hyalu- ronidase on anterior pituitary in oat w ere discussed .Immunocytochemical stainin identification w as con- ducted and row in characters evaluated.【Result】The results showed that the tissues presented best di- estion by 1 /L colla enase Ⅳplus 1 /L hy aluronidase, at 37 ℃2.5-3 h;anterior pituitary cells became adherent after 24 h of culture;the form of spindle or poly onal cells w ere found after 72 h of culture;they covered petri dish after 6 d of culture.Immunocytochemical stainin show ed that the cytoplasm of positive FSH cells show ed blue color, and the cytoplasm of PRL positive cells show ed blue brow n color,radioimmu- noassay showed secrete ac