左旋多巴合成研究进展.pdfVIP

2013 32 6 CHEMICAL INDUSTRY AND ENGINEERING PROGRESS 1367 300072 Q 815 A 100066132013 DOI 10.3969/j.issn.1000-6613.2013.06.028 Research progress in L-DOPA synthesis MA Qiangqiang ZHAO Guangrong Key Laboratory of Systems BioengineeringMinistry of EducationSchool of Chemical Engineering and Technology Tianjin UniversityTianjin 300072China Abstract L-dopa has been widely used in clinical treatment for Parkinsons disease. The methods for L-DOPA productionincluding total chemical synthesisextraction from plantsenzyme bioconversion and metabolic engineeringwere summarized in this review. The total chemical synthesis of L-dopa was complicate due to its asymmetric process and associated with certain environmental issues. Extracting L-dopa from plant suffered low yield and was limited by plant resource. Bioconversion of phenols to L-dopa has been catalyzed by enzymes tyrosine phenol lyasetyrosinase and transaminase. It is suggested that with the development of synthetic biologyfermentation of L-dopa by constructing metabolically engineered microorganisms would be a new high efficient and environment-friendly methodwhich demonstrates a promising process. Key words L-dopa total chemical synthesis plant extraction bioconversion metabolic engineering L-DOPA β-3,4- [3] -α-3,4-2dihydroxylphenylalanine[1(a)] 3--L