帕金森病MPTP模型小鼠两种行为学检测方法的比较研究_李丽波.pdfVIP

2009 30 15 ·1809 · · · MPTP 李丽波　王玉祥 【】　　在帕金森病M PTP 模型小鼠对爬杆法(pole test)和转棒法(rotarod test)两种行为 学检测方法进行 较研究。　C57/BL 小鼠腹腔注射 1-甲基-4-苯基1, 2, 3, 6-四氢吡啶 (MPTP)30mg/kg, 每天1 次, 连续5 天, 于第1、2、3、4、5 天分别用爬杆法和转棒法进行行为学检测。 　爬杆法的调头时间和爬下时间与小鼠的适应性行为有关, 且其评分标准易掺杂主观因素;而转棒 法是小鼠的被动运动, 潜伏期和掉落次数均为客观的观察指标。　与爬杆法相 , 转棒法是更为客 观的帕金森模型小鼠的行为学检测方法。 【】　1-甲基-4-苯基1, 2, 3, 6-四氢吡啶(MPTP)　帕金森病　行为学　爬杆法　转棒 法 A comparative study on two ethologyical methods in MPTP model mice of Parkinsons disease　L I L i -bo, et a l.　(Qiqihar M edical niversity, Heilongjiang 161006, China) 【Abstract】　Objective　To investigate the difference between pole test and rotarod test in MPTP model mice of Parkinsons disease.Methods　C57/BL mice were intraperitoneally injected (ip)1-Meth- yl-4-phenyl-1, 2, 3, 6-tetrahydro -pyridine(MPTP)30mg/kg once daily for consecutive five days st nd rd th and their behavior was examined in pole test and rotarod test on the 1 , 2 , 3 ,4 and 5th day.Results　 Subjective factor was inclined to be involved in pole test;indicators in rotarod test were more objective. Conclusions　Rotarod test is a more objective ethologyical method than pole test for MPTP model mice of Parkinsons disease. 【Key words】　Parkinsons disease　MPTP　Ethology 　Pole test　Rotarod test 　　(Parkinsons disease, PD) 2.5cm, 。, 10 [5] , /。: , 、, -, 。 1 cm, 60cm。。 、、。 1.1.3　　C57/BL , 22±2 g, , 1--4-1, 2, 3, 6 , :SCXK() -(1-Methyl-4-phenyl-1, 2, 3, 6-tetrahy- 2006-0009。 dropyridine, MPTP) 1.2　实验方法 [1-2] , 1.2.1　MPTP 　M PTP 。