张新祥《生化分析》酶的电化学 张淑冬 刘王莲.pptxVIP

酶固定化技术及 酶的生物传感器;;Fig. 1 Scheme of a biosensor.;固定化酶的优点：;酶固定化的载体：;Fig. 2 Schematic representation of the main different methods of enzyme immobilization. E: enzyme, P: inert protein.;包埋（entrapment）：; 聚糖：藻朊酸盐、琼脂 ;吸附（adsorption）：;分子间交联（cross-linking）：;Fig.4 Enzyme immobilization on carboxylated surface by carbodiimide coupling (A) without or (B) with NHS.;Fig. 5 Enzyme immobilization on an aminated surface by (A) carbodiimide or (B) glutaraldehyde coupling.;Fig. 6 Schematic representation of the reversible immobilization of histidine-tagged biomolecules to an electrogenerated poly(pyrrole)-