宫颈鳞癌组织转移相关基因的初步筛查.pdfVIP

中南大学学报(医学版) 20日， 38(4) ; h忧p:// JCent South Univ (Med S叫 405 回自抽回 画晶æ;l DOI:IO.3969/j.issn.1672-7347.20 13.04.012 菌嚣嚣 http://邮耶m.ne仙 宫颈鳞癌组织转移相关基因的初步筛查 吴宜林，邹浪，胡意，丁晖 (中南大学湘雅二医院妇产科，长沙41(011) [摘要]目的:应用基因芯片检测盆腔淋巴结转移组和无转移组宫颈鳞癌组织中差异表达基因，从中筛选肿瘤转 移相关基因。方法:利用基因芯片技术对4 例盆腔淋巴结转移组和6 例无转移组宫颈鳞癌组织标本进行差异表达基 因检测，并用 Real-time PCR 对部分差异表达基因进行验证。通过基因库检索、 PUBMED 文献检索和基因本体分析从 差异表达基因中筛选肿瘤转移相关基因。结果:从盆腔淋巴结转移组及无转移组宫颈鳞癌组织中筛选出表达差异明 显的基因 329 个，通过Real-time PCR验证，基因芯片结果可靠。从差异表达基因中筛选出与肿瘤转移相关基因44 个。 结论:通过基因芯片筛查，初步建立了宫颈鳞癌转移相关差异基因表达谱，说明宫颈鳞癌的转移过程受多基因调控。 [关键词]宫颈鳞癌;基因芯片;转移;差异表达基因 Prelimina叼screen of metastasis-related genes in cervical squamaous cell carcinoma WU Yilin, ZOU Lang, HU Yi, DING Hui (Department of Obstetrics and Gynecology,Second Xiangya Hospital, Central South Universi切 Changsha 410011, China) ABSTRACT Objective: To explore the differentially expressed gene profile in cervical squ缸nous cell carcinoma tissues with and without pelvic lymph node metastasis by microarray technique and to screen tumor metastasis-related genes. Methods: Cancer tissue samples were collected from 4 cervical squamous cell carcinoma patients with pelvic lymph metastasis and 6 patients without metastasis before the treatment. Differentially expressed gene profile was identified between cervical squamous cell carcinoma tissues with and without pelvic lymph node metastasis by NimbleGen microarray. Fluorescent real-time quantitative PCR was employed to validate the accuracy of the micro缸ray by detecting the expression of genes