Budding of Taenia crassiceps Cysticerci In Vitro Is Promoted by Crowding in Addition to Hormonal, Stress, and Energy-Related Signals英文文献资料.docVIP

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Budding of Taenia crassiceps Cysticerci In Vitro Is Promoted by Crowding in Addition to Hormonal, Stress, and Energy-Related Signals英文文献资料.doc

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Budding of Taenia crassiceps Cysticerci In Vitro Is Promoted by Crowding in Addition to Hormonal, Stress, and Energy-Related Signals英文文献资料

HindawiPublishingCorporation JournalofBiomedicineandBiotechnology Volume2010,ArticleID510978,5pages doi:10.1155/2010/510978 ResearchArticle BuddingofTaeniacrassicepsCysticerciInVitro IsPromotedbyCrowdinginAdditiontoHormonal, Stress,andEnergy-RelatedSignals PedroOstoa-Saloma,PedroOstoa-Jacobo,MarcelaEsquivel-Vel′azquez, SilvanaBazu′a,andCarlosLarralde InstitutodeInvestigacionesBiom′edicas,UniversidadNacionalAut′onomadeM′exico,A.P.70228,C.P.04510, M′exicoD.F.,Mexico CorrespondenceshouldbeaddressedtoPedroOstoa-Saloma,postoa@unam.mx Received19August2009;Accepted23October2009 AcademicEditor:JorgeMorales-Montor Copyright?2010PedroOstoa-Salomaetal.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttribution License,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperly cited. Taeniacrassicepscysticerci(cysts)reproducebybudding.Thecysts’productionofbudswasmeasuredinvitrotoexploreparasite andenvironmental-relatedfactorsinvolvedintheextremeindividualvariationinparasiteloadsofinbredmice.Cystswereplaced ininvitroculturefor10daysatinitialparasitedensitiesof1,5,10cysts/wellin1mlofRPMIMedium1640withoutserum.Results showedthatthereisconsiderableintrinsicinitialvariationamonginoculatedcystsintheirproductionofbudsandthatincreasing parasitedensity(crowding)stimulatestheoverallproductionofbudsandrecruitintobuddingmostofthecysts.Identicalcultures werethensubjectedtovarioustreatmentssuchasheatingandexposuretoperoxidetoinducestress,orto17?-estradiol,insulin, glucose,orinsulin+glucosetosupplementputativelylimitinghormonalandenergyresources.Alltreatmentsincreasedbudding buttheparasites’strongbuddingresponsetocrowdingaloneovershadowstheothertreatments. 1.Introduction genetical,immunological,endocrinological,andbehavioral studiesofhost-parasiterelationships[3–7]. Taenia crassiceps is a cestode that, when adult, lives in the intestinal lumen of some carnivore species (i.e., fox) andinthesubcutaneousconnectivetissueandpleuraland periton