三磷酸腺苷生物荧光法在急性髓系白血病细胞体外药物敏感性检测的初步应用.pdfVIP

中国药物警戒第 13 卷第 3 期 2016 年3 月 March ,2016 ,Vo I. 13,No.3 中圄分类号 :R969; R979.1 文献标识码 :A 文章编号: 1672-8629-(2016)02-00132-06 三磷酸腺昔生物荧光法在急性髓系白血病细胞体外药物敏感性检测 的初步应用 王版颖刘聪艳张维贺景娟苏力孙婉玲. (首都医科大学宣武医院血液科，北京 1【削53 ) 摘要 z 目的初步探索三磷酸腺昔生物荧光肿瘤体外药敏检测(ATP bioluminescence-tumor chemosensitivity ass勾， ATP-TCA) 技术在急性髓系白血病( acute myeloid leukemia, AML )中的临床应用价值。方法采集 18 例初治AML 患者的骨髓或外周血，分离单个核细胞，应用 ATP-TCA 技术，观察体外白血病细胞对化疗药物的敏感性，并分析 体外药敏结果、危险度分层及临床疗效三者之间的关系。结果 18 例初治AML 患者的白血病细胞在体外对8 种化 疗药物显示出不同的敏感性，有效率最高的是阿柔比星，无效率最高的是毗柔比星。在 14 例可进行疗效评价的初 治 AML 患者中观察到惠环类药物体外敏感性与临床疗效之间的完全相符率为78.6%，不同危险度分层患者之间的 临床疗效 (P=O.OI2 )和生存时间 (P=O.OI8 ) 均有显著差异，高危组患者生存时间最短。结论 AML 体外药敏检测 结果与临床治疗反应之间具有相关性趋势，ATP-TCA可能是开展AML个体化化疗的一种有效的体外药物筛选方法， 尤其是老年AML。 关键词:急性髓系白血病;药敏检测;三磷酸腺昔生物荧光法 Application of ATP Bioluminescence Assay in Chemosenstitivity of Acute Myeloid Leukemia Cells by ATP Bioluminescence in ~仿U WANG Yin-ying LIU Cong-yan ZHANG Wei HE Jing-juan SU Li SUN Wan-ling (Department of Hematology , Xuanwu Hospital, Capital Medical University , Beijing 1 仪)(}53， China) Abstract:O同ective To preliminarily explore the clinical value of ATP bioluminescence tumor chemosensitivity assay (ATP- TCA) in patients with acute myeloid leukemia (AML). Methods Peripheral blood or bone maπow mononuc1ear cells separated from newly diagnosed AML specimens were tested in vi/ro for cancer chemosensitivity to eight kinds of drugs by ATP-TCA, which was used for observing the susceptibility results ÚJ vitro and analyzing the relationships among the susc叩tibility results in vi /ro, risk stratification and clinical e伍cacy. Results Eighteen cases of AML patients displayed di能rent sensitivities to these eight kinds of 命ugs in vitro.Different drugs had different tumor growth inhibition ratio in vi.加， of which the highest efficiency is aclarubicin and the highest inefficiency is pirarubicin. Fourteen cases of de novo AML patients were evaluable for clinical efficacy. Entirely consistent rate b