DifferentialgeneexpressioninESPNETwithtype1vs在1型和espnet基因的差异表达.pptVIP

Sarcomas with Aberrant Transcription Factors: Biology and Expression Profiling Translocation-associated sarcomas General biological features and comparison to sarcomas with non-specific cytogenetic alterations Insights from microarray-based expression profiling of translocation-associated sarcomas Pathologic genetic rearrangements in human cancers: a family tree Molecular pathology of sarcomas: two major classes approx. 1/3 of all sarcomas 15 different sarcoma types with over 25 different translocations fusion genes: aberrant chimeric transcription factors (most) or aberrant kinases (some) biology: transcriptional deregulation or aberrant signaling Major fusion genes in sarcomas: biological overview Target Genes of Chimeric Transcription Factors in Sarcomas Slow progress in identifying genuine biologically critical target genes of chimeric transcription factors e.g. well-established targets: EWS-FLI1 15 EWS-WT1, PAX3-FKHR 5 each Low throughput gene-by-gene studies False leads generated by assays using exogenous target promoters and/or heterologous cells Most representative cellular background for inducible systems still subject of active investigation Need for more alternative higher throughput approaches Target Genes of Chimeric Transcription Factors in Selected Sarcomas Sarcomas with Aberrant Transcription Factors General biological features and comparison to sarcomas with non-specific cytogenetic alterations Insights from microarray-based expression profiling of translocation-associated sarcomas Expression Profiling of Sarcomas with Chimeric Transcription FactorsWhy is it interesting? Expression profiling of sarcomas with chimeric transcription factors Gene expression data analysis Hybridization to Affymetrix U133A GeneChip (22215 probe sets, 18500 transcripts, 14500 genes) Unsupervised Hierarchical Clustering Selection of differentially expressed genes Two-tailed t-tests; p0.01 after Bonferroni correction