A Mass Conserved Reaction–Diffusion System Captures Properties of Cell Polarity 英文参考文献.docVIP

AMassConservedReaction–DiffusionSystem CapturesPropertiesofCellPolarity Mikiya Otsuji1*,Shuji Ishihara2,Carl Co3,Kozo Kaibuchi4,Atsushi Mochizuki2,Shinya Kuroda5 1DepartmentofAnesthesiology,FacultyofMedicine,UniversityofTokyo,Tokyo,Japan,2DivisionofTheoreticalBiology,NationalInstituteforBasicBiology,Okazaki,Japan, 3DepartmentofCellularandMolecularPharmacologyandPrograminBiologicalSciences,UniversityofCaliforniaSanFrancisco,SanFrancisco,California,UnitedStatesof America,4Department ofCellPharmacology,Graduate School ofMedicine,Nagoya University, Nagoya,Japan, 5Department ofBiophysics andBiochemistry, Graduate SchoolofScience,UniversityofTokyo,Tokyo,Japan Cell polarity is a general cellular process that can be seen in various cell types such as migrating neutrophils and Dictyostelium cells. The Rho small GTP(guanosine 59-tri phosphate)ases have been shown to regulate cell polarity; however,itsmechanismofemergencehasyettobeclarified.Wefirstdevelopedareaction–diffusionmodeloftheRho GTPases,whichexhibitsswitch-likereversibleresponsetoagradientofextracellularsignals,exclusiveaccumulationof Cdc42andRac,orRhoAatthemaximalorminimalintensityofthesignal,respectively,andtrackingofchangesofa signalgradientbythepolarizedpeak.ThepreviouscellpolaritymodelsproposedbySubramanianandNarangshow similarbehaviorstoourRhoGTPasemodel,despitethedifferenceinmolecularnetworks.Thisledustocomparethese models,andwefoundthatthesemodelscommonlyshareinstabilityandamassconservationofcomponents.Basedon these common properties, we developed conceptual models of a mass conserved reaction–diffusion system with diffusion–driven instability. These conceptual models retained similar behaviors of cell polarity in the Rho GTPase model.Usingthesemodels,wenumericallyandanalyticallyfoundthatmultiplepolarizedpeaksareunstable,resulting in a single stable peak (uniqueness of axis), and that sensitivity toward changes of a signal gradient is specifically restrictedatthepolarizedpeak(localizedsensitivity).Alth