A Multi-Center Study to Evaluate the Performance of Phage Amplified Biologically Assay for Detecting TB in Sputum in the Pulmonary TB Patients 英文参考文献.docVIP

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A Multi-Center Study to Evaluate the Performance of Phage Amplified Biologically Assay for Detecting TB in Sputum in the Pulmonary TB Patients 英文参考文献.doc

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A Multi-Center Study to Evaluate the Performance of Phage Amplified Biologically Assay for Detecting TB in Sputum in the Pulmonary TB Patients 英文参考文献

AMulti-CenterStudytoEvaluatethePerformanceof PhageAmplifiedBiologicallyAssayforDetectingTBin SputuminthePulmonaryTBPatients ChangtaiZhu1,2,ZhenlingCui1,RuijuanZheng1,HuaYang1,RuiliangJin1,LianhuaQin1,ZhonghuaLiu1, JieWang1,ZhongyiHu1* 1ShanghaiKeyLaboratoryofTuberculosis,ShanghaiPulmonaryHospital,SchoolofMedicine,TongjiUniversity,Shanghai,People’sRepublicofChina,2Departmentof LaboratoryMedicine,ChangzhouTumorHospitalSoochowUniversity,Changzhou,People’sRepublicofChina Abstract Objective: To evaluate the performance of phage amplified biologically assay (PhaB) for detecting tuberculosis (TB) in sputuminthepulmonarytuberculosis(PTB)patients. Methods:ShanghaiTuberculosisKeyLaboratoryofShanghaiPulmonaryHospitalparticipatedintheprojectincollaboration withthelaboratoriesofsixhospitalsandatotalof1660eligibleparticipants(1351PTBpatientsand309non-TBpatients) were included in the study. The sputum samples from the participants were detected by smear microscopy, PhaB, and Lo¨wenstein-Jensen(L-J)culturemethod,respectively. Results:TheoverallsensitivityofPhaBwerehigherthanthatofL-Jcultureandsmearmicroscopy(p,0.05).Thesensitivity ofPhaBfordetectingsmear-negativespecimenswasobviouslyhigherthanthatofL-Jculture(p,0.05).ComparedwithL-J culture,theoverallsensitivity,specificity,PPV,NPV,ACCandKappavalueofPhaBwere98.4(95%Cl:96.9–99.3),71.6(95% Cl:68.4–74.6),67.7,98.7,81.7%and0.643,respectively.ThedetectionmediantimeofPhaBonlyneeded48hours,which wassignificantlylessthanthat(31days)ofL-Jculturemethod. Conclusion:PhaBmethodisarapidandsensitivemethodfordetectingTBinsputuminPTBpatients;especiallyforthe diagnosisofsmear-negativePTB,PhaBmethodisobviouslymoresensitivethanL-Jculturemethod. Citation:ZhuC,CuiZ,ZhengR,YangH,JinR,etal.(2011)AMulti-CenterStudytoEvaluatethePerformanceofPhageAmplifiedBiologicallyAssayforDetecting TBinSputuminthePulmonaryTBPatients.PLoSONE6(9):e24435.doi:10.1371/journal.pone.0024435 Editor:BradSpellberg,LosAngelesBiomedicalResearchInstitute,UnitedStatesofAmerica Receiv