A Multi-Analyte Assay for the Non-Invasive Detection of Bladder Cancer 英文参考文献.docVIP

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A Multi-Analyte Assay for the Non-Invasive Detection of Bladder Cancer 英文参考文献.doc

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A Multi-Analyte Assay for the Non-Invasive Detection of Bladder Cancer 英文参考文献

AMulti-AnalyteAssayfortheNon-InvasiveDetectionof BladderCancer SteveGoodison1,4,MyronChang2,YunfengDai2,VirginiaUrquidi1,4,CharlesJ.Rosser3,4 * 1CancerResearchInstitute,M.D.AndersonCancerCenterOrlando,Orlando,Florida,UnitedStatesofAmerica,2DepartmentofBiostatistics,TheUniversityofFlorida, Gainesville,Florida,UnitedStatesofAmerica,3SectionofUrologicOncology,MDAndersonCancerCenterOrlando,Orlando,Florida,UnitedStatesofAmerica,4Nonagen BioscienceCorp,Orlando,Florida,UnitedStatesofAmerica Abstract Accurateurinaryassaysforbladdercancer(BCa)detectionwouldbenefitbothpatientsandhealthcaresystems.Through genomic and proteomic profiling of urine components, we have previously identified a panel of biomarkers that can outperformcurrenturine-basedbiomarkersforthenon-invasivedetectionofBCa.Herein,wereportthediagnosticutilityof variousmultivariatecombinationsofthesebiomarkers.Weperformedacase-controlledvalidationstudyinwhichvoided urines from 127 patients (64 tumor bearing subjects) were analyzed. The urinary concentrations of 14 biomarkers (IL-8, MMP-9,MMP-10,SDC1,CCL18,PAI-1,CD44,VEGF,ANG,CA9,A1AT,OPN,PTX3,andAPOE)wereassessedbyenzyme-linked immunosorbentassay(ELISA).Diagnosticperformanceofeachbiomarkerandmultivariatemodelswerecomparedusing receiver operating characteristic curves and the chi-square test. An 8-biomarker model achieved the most accurate BCa diagnosis (sensitivity 92%, specificity 97%), but a combination of 3 of the 8 biomarkers (IL-8, VEGF, and APOE) was also highlyaccurate(sensitivity90%,specificity97%).Forcomparison,thecommercialBTA-TrakELISAtestachievedasensitivity of 79% and a specificity of 83%, and voided urine cytology detected only 33% of BCa cases in the same cohort. These datashowthatamultivariateurine-basedassaycanmarkedlyimprovetheaccuracyofnon-invasiveBCadetection.Further validationstudiesareunderwaytoinvestigatetheclinicalutilityofthispanelofbiomarkersforBCadiagnosisanddisease monitoring. Citation: Goodison S, Chang M, Dai Y, Urquidi V, Rosser CJ (2