A Novel Technique for the In Vivo Imaging of Autoimmune Diabetes Development in the Pancreas by Two-Photon Microscopy 英文参考文献.docVIP

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A Novel Technique for the In Vivo Imaging of Autoimmune Diabetes Development in the Pancreas by Two-Photon Microscopy 英文参考文献.doc

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A Novel Technique for the In Vivo Imaging of Autoimmune Diabetes Development in the Pancreas by Two-Photon Microscopy 英文参考文献

ANovelTechniquefortheInVivoImagingof AutoimmuneDiabetesDevelopmentinthePancreasby Two-PhotonMicroscopy KenCoppieters1,MarianneM.Martinic1¤,WilliamB.Kiosses2,NatalieAmirian1,MatthiasvonHerrath1* 1Type1Diabetes(T1D)CenterattheLaJollaInstituteforAllergyandImmunology,LaJolla,California,UnitedStatesofAmerica,2CoreMicroscopyFacility,TheScripps ResearchInstitute,LaJolla,California,UnitedStatesofAmerica Abstract Type1diabetes(T1D)ischaracterizedbytheimmune-mediateddestructionofbetacellsinthepancreas.Littleisknownabout theinvivodynamicinteractionsbetweenTcellsandbetacellsorthekineticbehaviorofotherimmunecellsubsetsinthe pancreaticislets.Utilizingmultiphotonmicroscopywehavedesignedatechniquethatallowsforthereal-timevisualizationof diabetogenicTcellsanddendriticcellsinpancreaticisletsinaliveanimal,includingtheirinterplaywithbetacellsandthe vasculature.Usingacustomdesignedstage,thepancreaswassurgicallyexposedunderliveconditionssothatimagingof isletsunderintactbloodpressureandoxygensupplybecamepossible.Wedemonstrateherethatthisapproachallowsforthe trackingofdiabetogenicleukocytesaswellasvascularizationphenotypeofisletsandaccumulationofdendriticcellsinislets duringdiabetespathogenesis.ThistechniqueshouldbeusefulinmappingcrucialkineticeventsinT1Dpathogenesisandin testingtheimpactofimmunebasedinterventionsonTcellmigration,extravasationandisletdestruction. Citation: Coppieters K, Martinic MM, Kiosses WB, Amirian N, von Herrath M (2010) A Novel Technique for the In Vivo Imaging of Autoimmune Diabetes DevelopmentinthePancreasbyTwo-PhotonMicroscopy.PLoSONE5(12):e15732.doi:10.1371/journal.pone.0015732 Editor:KathrinMaedler,UniversityofBremen,Germany ReceivedAugust19,2010;AcceptedNovember23,2010;PublishedDecember23,2010 Copyright: ?2010Coppieters etal.This isanopen-accessarticledistributed underthe terms ofthe Creative Commons AttributionLicense, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:JuvenileDiabe