A Nuclear Family A DNA Polymerase from Entamoeba histolytica Bypasses Thymine Glycol 英文参考文献.docVIP

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A Nuclear Family A DNA Polymerase from Entamoeba histolytica Bypasses Thymine Glycol 英文参考文献.doc

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A Nuclear Family A DNA Polymerase from Entamoeba histolytica Bypasses Thymine Glycol 英文参考文献

ANuclearFamilyADNAPolymerasefromEntamoeba histolyticaBypassesThymineGlycol GuillermoPastor-Palacios1,ElisaAzuara-Liceaga2,LuisG.Brieba1* 1Laboratorio Nacional de Geno′mica para la Biodiversidad, CINVESTAV, Irapuato, Me′xico, 2Posgrado en Ciencias Geno′micas, Universidad Auto′noma de la Ciudad de Me′xico,Me′xicoDistritoFederal,Me′xico Abstract Background: Eukaryotic family A DNA polymerases are involved in mitochondrial DNA replication or translesion DNA synthesis. Here, we present evidence that the sole family A DNA polymerase from the parasite protozoan E. histolytica (EhDNApolA) localizes to the nucleus and that its biochemical properties indicate that this DNA polymerase may be involvedintranslesionDNAsynthesis. Methodology and Results: EhDNApolA is the sole family A DNA polymerase in E. histolytica. An in silico analysis places familyADNApolymerasesfromthegenusEntamoebainaseparatebranchofafamilyADNApolymerasesphylogenetic tree. Biochemical studies of a purified recombinant EhDNApolA demonstrated that this polymerase is active in primer elongation,ispoorlyprocessive,displaysmoderatestranddisplacement,anddoesnotcontain39–59exonucleaseorediting activity.Importantly,EhDNApolAbypassesthymineglycollesionswithhighfidelity,andconfocalmicroscopydemonstrates thatthispolymeraseistranslocatedintothenucleus.ThesedatasuggestaputativeroleofEhDNApolAintranslesionDNA synthesisinE.histolytica. Conclusion:ThisisthefirstreportofthebiochemicalcharacterizationofaDNApolymerasefromE.histolytica.EhDNApolAis a family A DNA polymerase that is grouped into a new subfamily of DNA polymerases with translesion DNA synthesis capabilitiessimilartoDNApolymerasesfromsubfamilyn. Citation:Pastor-PalaciosG,Azuara-LiceagaE,BriebaLG(2010)ANuclearFamilyADNAPolymerasefromEntamoebahistolyticaBypassesThymineGlycol.PLoS NeglTropDis4(8):e786.doi:10.1371/journal.pntd.0000786 Editor:AnuradhaLohia,BoseInstitute,India ReceivedJanuary19,2010;AcceptedJuly12,2010;PublishedAugust10,2010 Copyright:?2010Pastor-Palaci