A Pol V–Mediated Silencing, Independent of RNA–Directed DNA Methylation, Applies to 5S rDNA 英文参考文献.docVIP

下载本文档

0
0
约3.91万字
约 8页
2017-05-11 发布于上海
举报
版权申诉

A Pol V–Mediated Silencing, Independent of RNA–Directed DNA Methylation, Applies to 5S rDNA 英文参考文献.doc

1、本文档共8页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

A Pol V–Mediated Silencing, Independent of RNA–Directed DNA Methylation, Applies to 5S rDNA 英文参考文献

APolV–MediatedSilencing,Independentof RNA–DirectedDNAMethylation,Appliesto5SrDNA JulienDouet,SylvieTutois,SylvetteTourmente* CNRS,UMR6247GReD,ClermontUniversite′, INSERMU931,Aubie`re, France Abstract Theplant-specificRNApolymerasesPolIVandPolVareessentialtoRNA–directedDNAmethylation(RdDM),whichalso requires activities from RDR2 (RNA–Dependent RNA Polymerase 2), DCL3 (Dicer-Like 3), AGO4 (Argonaute), and DRM2 (Domains Rearranged Methyltransferase 2). RdDM is dedicated to the methylation of target sequences which include transposableelements,regulatoryregionsofseveralprotein-codinggenes,and5SrRNA–encodingDNA(rDNA)arrays.In thispaper,wehavestudiedtheexpressionofthe5S-210transcript,amarkerofsilencingreleaseat5SRNAgenes,toshowa differential impact of RNA polymerases IV and V on 5S rDNA arrays during early development of the plant. Using a combination of molecular and cytological assays, we show that Pol IV, RDR2, DRM2, and Pol V, actors of the RdDM, are required to maintain a transcriptional silencing of 5S RNA genes at chromosomes 4 and 5. Moreover, we have shown a derepressionassociatedtochromatindecondensationspecifictothe5Sarrayfromchromosome4andrestrictedtothePol V–lossoffunction.Inconclusion,ourresultshighlightanewroleforPolVon5SrDNA,whichisRdDM–independentand comesspecificallyatchromosome4,inadditiontotheRdDMpathway. Citation: Douet J, Tutois S, Tourmente S (2009) A Pol V–Mediated Silencing, Independent of RNA–Directed DNA Methylation, Applies to 5S rDNA. PLoS Genet5(10):e1000690.doi:10.1371/journal.pgen.1000690 Editor:JosephR.Ecker,TheSalkInstituteforBiologicalStudies,UnitedStatesofAmerica ReceivedMarch10,2009;AcceptedSeptember17,2009;PublishedOctober16,2009 Copyright: ? 2009 Douet et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding: This work was supported by the Centre Nation