A Ranking System for Reference Libraries of DNA Barcodes Application to Marine Fish Species from Portugal 英文参考文献.docVIP

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A Ranking System for Reference Libraries of DNA Barcodes Application to Marine Fish Species from Portugal 英文参考文献.doc

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A Ranking System for Reference Libraries of DNA Barcodes Application to Marine Fish Species from Portugal 英文参考文献

ARankingSystemforReferenceLibrariesofDNA Barcodes:ApplicationtoMarineFishSpeciesfrom Portugal FilipeO.Costa1*,MonicaLandi1,RogeliaMartins2,MariaH.Costa3,MariaE.Costa4,MiguelCarneiro2, MariaJ.Alves5,DirkSteinke6,GaryR.Carvalho7 1CBMA(CentreofMolecularandEnvironmentalBiology),DepartmentofBiology,UniversityofMinho,CampusdeGualtar,Braga,Portugal,2INRB,IP/IPIMAR, Lisboa, Portugal,3IMAR–InstitutodoMar,DepartamentodeCie?nciaseEngenhariadoAmbiente,FaculdadedeCie?nciaseTecnologia,UniversidadeNovadeLisboa,Caparica, Portugal, 4Centro de Cie?ncias do Mar (CCMAR), Universidade do Algarve, Faculdade de Cie?ncias e Tecnologias (FCT), Campus de Gambelas, Faro, Portugal, 5Museu NacionaldeHisto′riaNaturalCentrodeBiologiaAmbiental,UniversidadedeLisboa,Lisboa,Portugal,6BiodiversityInstituteofOntarioUniversityofGuelph,Guelph, Ontario,Canada,7MolecularEcologyandFisheriesGeneticsLaboratory,SchoolofBiologicalSciences,BangorUniversity,Bangor,Gwynedd,Wales Abstract Background:TheincreasingavailabilityofreferencelibrariesofDNAbarcodes(RLDB)offerstheopportunitytothescreen thelevelofconsistencyinDNAbarcodedataamonglibraries,inordertodetectpossibledisagreementsgeneratedfrom taxonomicuncertaintyoroperationalshortcomings.Weproposearankingsystemtoattributeaconfidenceleveltospecies identificationsassociatedwithDNAbarcoderecordsfromaRLDB.Hereweapplytheproposedrankingsystemtoanewly generatedRLDBformarinefishofPortugal. Methodology/Principal Findings: Specimens (n=659) representing 102 marine fish species were collected along the continentalshelfofPortugal,morphologicallyidentifiedandarchivedinamuseumcollection.Samplesweresequencedat thebarcoderegionofthecytochromeoxidasesubunitIgene(COI-5P).ResultantDNAbarcodeshadaverageintra-specific and inter-specific Kimura-2-parameter distances (0.32% and 8.84%, respectively) within the range usually observed for marinefishes.Allspecimenswererankedinfivedifferentlevels(A–E),accordingtothereliabilityofthematchbetweentheir species identification and the