A Single Molecule Scaffold for the Maize Genome 英文参考文献.docVIP

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A Single Molecule Scaffold for the Maize Genome 英文参考文献.doc

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A Single Molecule Scaffold for the Maize Genome 英文参考文献

ASingleMoleculeScaffoldfortheMaizeGenome ShiguoZhou1,FushengWei2,JohnNguyen3,MikeBechner1,KonstantinosPotamousis1 ,Steve Goldstein1,LouisePape1,MichaelR.Mehan3,ChrisChuras1,ShiranPasternak4,DanK.Forrest1, RogerWise5,DoreenWare4,6,RodA.Wing2,MichaelS.Waterman3,MironLivny7,DavidC.Schwartz1* 1LaboratoryforMolecularandComputationalGenomics,DepartmentofChemistry,LaboratoryofGenetics,UWBiotechnologyCenter,UniversityofWisconsin–Madison, Madison, Wisconsin, United States of America, 2Department of Plant Sciences, Arizona Genomics Institute, University of Arizona, Tucson, Arizona, United States of America,3DepartmentsofMathematics,Biology,andComputerScience,UniversityofSouthernCalifornia,LosAngeles,California,UnitedStatesofAmerica,4ColdSpring Harbor Laboratory, Cold Spring Harbor, New York, United States of America, 5Corn Insects and Crop Genetics Research, United States Department of Agriculture– AgriculturalResearchServiceandDepartmentofPlantPathology,IowaStateUniversity,Ames,Iowa,UnitedStatesofAmerica,6Plant,Soil,andNutritionResearch,United StatesDepartmentofAgriculture–AgriculturalResearchService,Ithaca,NewYork,UnitedStatesofAmerica,7ComputerSciencesDepartment,UniversityofWisconsin- Madison,Madison,Wisconsin,UnitedStatesofAmerica Abstract About85%ofthemaizegenomeconsistsofhighlyrepetitivesequencesthatareinterspersedbylow-copy,gene-coding sequences.Themaizecommunityhasdealtwiththisgenomiccomplexitybytheconstructionofanintegratedgeneticand physicalmap(iMap),butthisresourcealonewasnotsufficientforensuringthequalityofthecurrentsequencebuild.For thispurpose,weconstructedagenome-wide,high-resolutionopticalmapofthemaizeinbredlineB73genomecontaining .91,000 restriction sites (averaging 1 site/,23kb) accrued from mapping genomic DNA molecules. Our optical map comprises66contigs,averaging31.88Mbinsizeandspanning91.5%(2,103.93Mb/,2,300Mb)ofthemaizegenome.A new algorithm was created that considered both optical map and unfinished BAC sequence data for placing 60/66 (2,032.42Mb) op