A Topological Map of the Compartmentalized Arabidopsis thaliana Leaf Metabolome 英文参考文献.docVIP

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A Topological Map of the Compartmentalized Arabidopsis thaliana Leaf Metabolome 英文参考文献.doc

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A Topological Map of the Compartmentalized Arabidopsis thaliana Leaf Metabolome 英文参考文献

ATopologicalMapoftheCompartmentalized ArabidopsisthalianaLeafMetabolome StephanKrueger1.,PatrickGiavalisco2.,LeonardKrall2,Marie-CarolineSteinhauser2,DirkBu¨ssis3, BjoernUsadel2,Ulf-IngoFlu¨gge1,AlisdairR.Fernie2,LotharWillmitzer2,DirkSteinhauser2*. 1BotanicalInstitute,UniversityofCologne,Cologne,Germany,2MaxPlanckInstituteofMolecularPlantPhysiology,Potsdam-Golm,Germany,3GABIManagingOffice, c/oMaxPlanckInstituteofMolecularPlantPhysiology,Potsdam-Golm,Germany Abstract Background:Theextensivesubcellularcompartmentalizationofmetabolitesandmetabolismineukaryoticcellsiswidely acknowledged and represents a key factor of metabolic activity and functionality. In striking contrast, the knowledge of actual compartmental distribution of metabolites from experimental studies is surprisingly low. However, a precise knowledgeof,possiblyall,metabolitesandtheirsubcellulardistributionsremainsakeyprerequisitefortheunderstanding ofanycellularfunction. Methodology/Principal Findings: Here we describe results for the subcellular distribution of 1,117 polar and 2,804 lipophilic mass spectrometric features associated to known and unknown compounds from leaves of the model plant Arabidopsisthaliana.Usinganoptimizednon-aqueousfractionationprotocolinconjunctionwithGC/MS-andLC/MS-based metaboliteprofiling,81.5%ofthemetabolicdatacouldbeassociatedtooneofthreesubcellularcompartments:thecytosol (includingthemitochondria),vacuole,orplastids.Statisticalanalysisusingamarker-‘free’approachrevealedthat18.5%of thesemetabolitesshowintermediatedistributions,whichcaneitherbeexplainedbytransportprocessesorbyadditional subcellularcompartments. Conclusion/Significance:Nexttoafunctionalandconceptualworkflowfortheefficient,highlyresolvedmetaboliteanalysis of the fractionated Arabidopsis thaliana leaf metabolome, a detailed survey of the subcellular distribution of several compounds, in the graphical format of a topological map, is provided. This complex data set therefore does not only contain a rich reposit