A Type 2C Protein Phosphatase FgPtc3 Is Involved in Cell Wall Integrity, Lipid Metabolism, and Virulence in Fusarium graminearum 英文参考文献.docVIP

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A Type 2C Protein Phosphatase FgPtc3 Is Involved in Cell Wall Integrity, Lipid Metabolism, and Virulence in Fusarium graminearum 英文参考文献.doc

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A Type 2C Protein Phosphatase FgPtc3 Is Involved in Cell Wall Integrity, Lipid Metabolism, and Virulence in Fusarium graminearum 英文参考文献

AType2CProteinPhosphataseFgPtc3IsInvolvedinCell WallIntegrity,LipidMetabolism,andVirulencein Fusariumgraminearum JinhuaJiang1,YingziYun1,QianqianYang1,Won-BoShim2,ZhengyiWang1,ZhonghuaMa1* 1KeyLaboratoryofMolecularBiologyofCropPathogensandInsects,InstituteofBiotechnology,ZhejiangUniversity,Hangzhou,China,2DepartmentofPlantPathology andMicrobiology,TexasAMUniversity,CollegeStation,Texas,UnitedStatesofAmerica Abstract Type2Cproteinphosphatases(PP2Cs)playimportantrolesinregulatingmanybiologicalprocessesineukaryotes.Currently, littleisknownaboutfunctionsofPP2Csinfilamentousfungi.Thecausalagentofwheatheadblight,Fusariumgraminearum, contains seven putative PP2C genes, FgPTC1, -3, -5, -5R, -6, -7 and -7R. In order to investigate roles of these PP2Cs, we constructed deletion mutants for all seven PP2C genes in this study. The FgPTC3 deletion mutant (DFgPtc3-8) exhibited reducedaerialhyphaeformationanddeoxynivalenol(DON)production,butincreasedproductionofconidia.Themutant showedincreasedresistancetoosmoticstressandcellwall-damagingagentsonpotatodextroseagarplates.Pathogencity assaysshowedthatDFgPtc3-8isunabletoinfectfloweringwheathead.AllofthedefectswererestoredwhenDFgPtc3-8 was complemented withthe wild-typeFgPTC3 gene. Additionally, theFgPTC3 partially rescued growth defect of ayeast PTC1deletionmutant undervarious stressconditions. Ultrastructural andhistochemical analysesshowedthat conidia of DFgPtc3-8containedanunusuallyhighnumberoflargelipiddroplets.Furthermore,themutantaccumulatedahigherbasal levelofglycerolthanthewild-typeprogenitor.Quantitativereal-timePCRassaysshowedthatbasalexpressionofFgOS2, FgSLT2 and FgMKK1 in the mutant was significantly higher than that in the wild-type strain. Serial analysis of gene expression in DFgPtc3-8 revealed that FgPTC3 is associated with various metabolic pathways. In contrast to the FgPTC3 mutant,thedeletionmutantsofFgPTC1,FgPTC5,FgPTC5R,FgPTC6,FgPTC7orFgPTC7Rdidnotshowaberrantphenotypic featureswhengrownonPDAmediumorinoculate