Activation of DNA-PK by Ionizing Radiation Is Mediated by Protein Phosphatase 6 英文参考文献.docVIP

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Activation of DNA-PK by Ionizing Radiation Is Mediated by Protein Phosphatase 6 英文参考文献.doc

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Activation of DNA-PK by Ionizing Radiation Is Mediated by Protein Phosphatase 6 英文参考文献

ActivationofDNA-PKbyIonizingRadiationIsMediated byProteinPhosphatase6 JunMi1,JaroslawDziegielewski1,ElzbietaBolesta1,DavidL.Brautigan2,JamesM.Larner1* 1Department of Radiation Oncology, University of Virginia Health System, Charlottesville, Virginia, United States of America, 2Center for Cell Signaling,University of VirginiaHealthSystem,Charlottesville,Virginia,UnitedStatesofAmerica Abstract DNA-dependent protein kinase (DNA-PK) plays a critical role in DNA damage repair, especially in non-homologous end- joiningrepairofdouble-strandbreakssuchasthoseformedbyionizingradiation(IR)inthecourseofradiationtherapy. Regulation of DNA-PK involves multisite phosphorylation but this is incompletely understood and little is known about protein phosphatases relative to DNA-PK. Mass spectrometry analysis revealed that DNA-PK interacts with the protein phosphatase-6(PP6)SAPSsubunitPP6R1.PP6isaheterotrimericenzymethatconsistsofacatalyticsubunit,plusoneof threePP6SAPSregulatorysubunitsandoneofthreeankyrinrepeatsubunits.EndogenousPP6R1co-immunoprecipitated DNA-PK,andIRenhancedtheamountofcomplexandpromoteditsimportintothenucleus.Inaddition,siRNAknockdown ofeitherPP6R1orPP6significantlydecreasedIRactivationofDNA-PK,suggestingthatPP6activatesDNA-PKbyassociation anddephosphorylation.KnockdownofotherphosphatasesPP5orPP1c1andsubunitsPP6R3orARS-AdidnotreduceIR activation of DNA-PK, demonstrating specificity for PP6R1. Finally, siRNA knockdown of PP6R1 or PP6 but not other phosphatases increased the sensitivity of glioblastoma cells to radiation-induced cell death to a level similar to DNA-PK deficient cells. Our data demonstrate that PP6 associates with and activates DNA-PK in response to ionizing radiation. Therefore,thePP6/PP6R1phosphataseisapotentialmoleculartargetforradiationsensitizationbychemicalinhibition. Citation:MiJ,DziegielewskiJ,BolestaE,BrautiganDL,LarnerJM(2009)ActivationofDNA-PKbyIonizingRadiationIsMediatedbyProteinPhosphatase6.PLoS ONE4(2):e4395.doi:10.1371/journal.pone.00