Analysis of a Splice Array Experiment Elucidates Roles of Chromatin Elongation Factor Spt4–5 in Splicing 英文参考文献.docVIP
- 1、本文档共13页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
- 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
- 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们。
- 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Analysis of a Splice Array Experiment Elucidates Roles of Chromatin Elongation Factor Spt4–5 in Splicing 英文参考文献
AnalysisofaSpliceArrayExperiment
ElucidatesRolesofChromatinElongation
FactorSpt4–5inSplicing
Yuanyuan Xiao1[,Yee H.Yang2[,Todd A.Burckin3,Lily Shiue4,Grant A.Hartzog3,Mark R.Segal1*
1 Department of Epidemiology and Biostatistics, Center for Bioinformatics and Molecular Biostatistics, University of California, San Francisco, California, United States of
America, 2 Department of Medicine, Center for Bioinformatics and Molecular Biostatistics, University of California, San Francisco, California, United States of America, 3
DepartmentofMolecular,Cell,andDevelopmentalBiology,UniversityofCalifornia,SantaCruz,California,UnitedStatesofAmerica,4DepartmentofMolecular,Celland
DevelopmentalBiology,UniversityofCalifornia,SantaCruz,California,UnitedStatesofAmerica
Splicingisanimportantprocessforregulationofgeneexpressionineukaryotes,andithasimportantfunctionallinks
tootherstepsofgeneexpression.TwoexamplesoftheselinkagesincludeCeg1,acomponentofthemRNAcapping
enzyme,andthechromatinelongationfactorsSpt4–5,bothofwhichhaverecentlybeenshowntoplayaroleinthe
normalsplicingofseveralgenesintheyeastSaccharomycescerevisiae.Usingagenomicapproachtocharacterizethe
rolesofSpt4–5insplicing,weusedsplicing-sensitiveDNAmicroarraystoidentifyspecificsetsofgenesthataremis-
splicedinceg1,spt4,andspt5mutants.Inthecontextofacomplex,nested,experimentaldesignfeaturing22dye-swap
arrayhybridizations,comprisingbothbiologicalandtechnicalreplicates,weappliedfiveappropriatestatisticalmodels
forassessingdifferentialexpressionbetweenwild-typeandthemutants.Torefineselectionofdifferentialexpression
genes,wethenusedarobustmodel-synthesizingapproach,DifferentialExpressionviaDistanceSynthesis,tointegrate
all five models. The resultant list of differentially expressed genes was then further analyzed with regard to select
attributes: we found that highly transcribed genes with long introns were most sensitive to spt mutations. QPCR
confirmationofdifferentialexpressionwasestablishedforthelimitednumberofgenesevaluated
您可能关注的文档
- An Integrative Multi-Network and Multi-Classifier Approach to Predict Genetic Interactions 英文参考文献.doc
- An Integrative Method for Accurate Comparative Genome Mapping 英文参考文献.doc
- An Integrated Transcriptomic and Meta-Analysis of Hepatoma Cells Reveals Factors That Influence Susceptibility to HCV Infection 英文参考文献.doc
- An Integrin from Shrimp Litopenaeus vannamei Mediated Microbial Agglutination and Cell Proliferation 英文参考文献.doc
- An Integrated Strategy to Study Muscle Development and Myofilament Structure in Caenorhabditis elegans 英文参考文献.doc
- An Integrative -omics Approach to Identify Functional Sub-Networks in Human Colorectal Cancer 英文参考文献.doc
- An Intermittent Live Cell Imaging Screen for siRNA Enhancers and Suppressors of a Kinesin-5 Inhibitor 英文参考文献.doc
- An Interactive Internet-Based Continuing Education Course on Sexually Transmitted Diseases for Physicians and Midwives in Peru 英文参考文献.doc
- An Intergenic Non-Coding rRNA Correlated with Expression of the rRNA and Frequency of an rRNA Single Nucleotide Polymorphism in Lung Cancer Cells 英文参考文献.doc
- An Interaction Network Predicted from Public Data as a Discovery Tool Application to the Hsp90 Molecular Chaperone Machine 英文参考文献.doc
文档评论(0)