Analysis of Allogenicity of Mesenchymal Stem Cells in Engraftment and Wound Healing in Mice 英文参考文献.docVIP
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Analysis of Allogenicity of Mesenchymal Stem Cells in Engraftment and Wound Healing in Mice 英文参考文献
AnalysisofAllogenicityofMesenchymalStemCellsin
EngraftmentandWoundHealinginMice
LiwenChen1,3,EdwardE.Tredget3,ChenxiongLiu2,YaojiongWu2,3*
1CollegeofAnimalScience,SouthChinaAgriculturalUniversity,Wushan,Guangzhou,China,2LifeScienceDivision,TsinghuaUniversityGraduateSchoolatShenzhen,
TheUniversityTown,Shenzhen,China,3DepartmentofSurgery,DepartmentofBiochemistry,UniversityofAlberta,Edmonton,Alberta,Canada
Abstract
Studieshaveshownthatallogeneic(allo-)bonemarrowderivedmesenchymalstemcells(BM-MSCs)mayenhancetissue
repair/regeneration. However, recent studies suggest that immune rejection may occur to allo-MSCs leading to reduced
engraftment. In this study, we compared allo-BM-MSCs with syngeneic BM-MSCs or allo-fibroblasts in engraftment and
effect in wound healing. Equal numbers of GFP-expressing allo-BM-MSCs, syngeneic BM-MSCs or allo-fibroblasts were
implantedintoexcisionalwoundsinGFP-negativemice.QuantificationofGFP-expressingcellsinwoundsat7,14and28
daysindicatedsimilaramountsofallogeneicorsyngeneicBM-MSCsbutsignificantlyreducedamountsofallo-fibroblasts.
Withhealingprogression,decreasingamountsofallogeneicandsyngeneicBM-MSCswerefoundinthewound;however,
the reduction was more evident (2 fold) in allo-fibroblasts. Similar effects in enhancing wound closure were found in
allogeneicandsyngeneicBM-MSCsbutnotinallo-fibroblasts.Histologicalanalysisshowedthatallo-fibroblastswerelargely
confinedtotheinjectionsiteswhileallo-BM-MSCshadmigratedintotheentirewound.Quantificationofinflammatorycells
+
inwoundsshowedthatallo-fibroblast-butnotallo-BM-MSC-treatedwoundshadsignificantlyincreasedCD45 leukocytes,
CD3 lymphocytesandCD8 Tcells.OurstudysuggeststhatallogeneicBM-MSCsexhibitignorableimmunogenicityandare
equallyefficientassyngeneicBM-MSCsinengraftmentandinenhancingwoundhealing.
+
+
Citation:ChenL,TredgetEE,LiuC,WuY(2009)AnalysisofAllogenicityofMesenchymalStemCellsinEngraftmentandWoundHealinginMice.PLoSONE4(9):
e7119.doi:10.1371/journal.pone.0007119
Editor:JacquesZimmer
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