Aβ Peptide Fibrillar Architectures Controlled by Conformational Constraints of the Monomer 英文参考文献.docVIP

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Aβ Peptide Fibrillar Architectures Controlled by Conformational Constraints of the Monomer 英文参考文献.doc

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Aβ Peptide Fibrillar Architectures Controlled by Conformational Constraints of the Monomer 英文参考文献

AbPeptideFibrillarArchitecturesControlledby ConformationalConstraintsoftheMonomer KristofferBra¨nnstro¨m1,AndersOhman¨ 2*,AndersOlofsson1* 1DepartmentofMedicalBiochemistryandBiophysics,Umea? University,Umea?, Sweden,2DepartmentofChemistry,Umea? University,Umea?, Sweden Abstract Anomalousself-assemblyoftheAbpeptideintofibrillaramyloiddepositsisstronglycorrelatedwiththedevelopmentof Alzheimer’s disease. Ab fibril extension follows a template guided ‘‘dock and lock’’ mechanism where polymerisation is catalysedbythefibrillarends.Usingsurfaceplasmonresonance(SPR)andquenchedhydrogen-deuteriumexchangeNMR (H/D-exchangeNMR),wehaveanalysedthefibrillarstructureandpolymerisationpropertiesofboththehighlyaggregation prone Ab1–40 Glu22Gly (Ab40Arc) and wild type Ab1–40 (Ab40WT). The solvent protection patterns from H/D exchange experimentssuggestverysimilarstructuresofthefibrillarforms.However,throughcross-seedingexperimentsmonitored bySPR,wefoundthatthemonomericformofAb40WTissignificantlyimpairedtoacquirethefibrillararchitectureofAb40Arc . A detailed characterisation demonstrated that Ab40WT has a restricted ability to dock and isomerise with high binding affinity onto Ab40Arc fibrils. These results have general implications for the process of fibril assembly, where the rate of polymerisation,andconsequentlythearchitectureoftheformedfibrils,isrestrictedbyconformationalconstraintsofthe monomers.Interestingly,wealsofoundthatthekineticrateoffibrilformationratherthanthethermodynamicallylowest energystatedeterminestheoverallfibrillarstructure. Citation:Bra¨nnstro¨mK,O¨hmanA,OlofssonA(2011)AbPeptideFibrillarArchitecturesControlledbyConformationalConstraintsoftheMonomer.PLoSONE6(9): e25157.doi:10.1371/journal.pone.0025157 Editor:EdatharaAbraham,UniversityofArkansasforMedicalSciences,UnitedStatesofAmerica ReceivedJune9,2011;AcceptedAugust26,2011;PublishedSeptember29,2011 Copyright:?2011Bra¨nnstro¨metal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommon