Biglycan Deletion Alters Adiponectin Expression in Murine Adipose Tissue and 3T3-L1 Adipocytes 英文参考文献.docVIP

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Biglycan Deletion Alters Adiponectin Expression in Murine Adipose Tissue and 3T3-L1 Adipocytes 英文参考文献.doc

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Biglycan Deletion Alters Adiponectin Expression in Murine Adipose Tissue and 3T3-L1 Adipocytes 英文参考文献

BiglycanDeletionAltersAdiponectinExpressionin MurineAdiposeTissueand3T3-L1Adipocytes MelizaG.Ward,KolapoM.Ajuwon* DepartmentofAnimalSciences,PurdueUniversity,WestLafayette,Indiana,UnitedStatesofAmerica Abstract Obesity promotes increased secretion of a number of inflammatory factors from adipose tissue. These factors include cytokinesandverylately,extracellularmatrixcomponents(ECM).Biglycan,asmallleucinerichproteoglycanECMprotein,is up-regulatedinobesityandhasrecentlybeenrecognizedasapro-inflammatorymolecule.However,itisunknownwhether biglycancontributestoadiposetissuedysfunction.Inthepresentstudy,wecharacterizedbiglycanexpressioninvarious adiposedepotsinwild-typemicefedalowfatdiet(LFD)orobesity-inducinghighfatdiet(HFD).Highfatfeedinginduced biglycan mRNA expression in multiple adipose depots. Adiponectin is an adipokine with anti-inflammatory and insulin sensitizingeffects.Duetotheimportanceofadiponectin,weexaminedtheeffectofbiglycanonadiponectinexpression. Comparison of adiponectin expression in biglycan knockout (bgn2/0) and wild-type (bgn+/0) reveals higher adiponectin mRNAandproteininepididymalwhiteadiposetissueinbgn2/0mice,aswellhigherserumconcentrationofadiponectin, and lower serum insulin concentration. On the contrary, knockdown of biglycan in 3T3-L1 adipocytes led to decreased expression and secretion of adiponectin. Furthermore, treatment of 3T3-L1 adipocytes with conditioned medium from biglycantreatedmacrophagesresultedinanincreaseinadiponectinmRNAexpression.Thesedatasuggestalinkbetween biglycan and adiponectin expression. However, the difference in the pattern of regulation between invivo and invitro settingsrevealsthecomplexityofthisrelationship. Citation:WardMG,AjuwonKM(2012)BiglycanDeletionAltersAdiponectinExpressioninMurineAdiposeTissueand3T3-L1Adipocytes.PLoSONE7(11):e50554. doi:10.1371/journal.pone.0050554 Editor:MakotoKanzaki,TohokuUniversity,Japan ReceivedAugust20,2012;AcceptedOctober24,2012;PublishedNovember26,2012 Copyright: ? 2012 Ward, Aj