ACA液芯微胶囊制备工艺优化.pdfVIP

学兔兔 化 工 进 展 · 124 CHEMICAL INDUSTRY AND ENGINEERING PROGRESS 20 1 0年第29卷第1期 ACA液芯微胶囊制备工艺优化 朱敏莉，薛伟明，赵彬然，刘 博，谢晓环 (西北大学化工学院，陕西 西安 71O069) 摘 要：采用响应面法优化工艺条件，制备具有较好机械强度的海藻酸盐一壳聚糖-海藻酸盐(ACA)液芯微胶囊。 首先，通过Plackett—Burman方法对相关影响因素的效应进行评价，筛选出有显著效应的壳聚糖浓度、壳聚糖溶液pH 值和柠檬酸钠溶液pH值3个因素；其次，采用最陡爬坡实验接近具有最好膜强度的中心点区域；最后由中心组合 实验和响应面分析确定主要影响因子的最佳条件。在优4~：r-艺条件下验证膜的机械强度，膨胀率一14．62％与优化结 果一15．41％接近，优化结果与实际情况较好吻合。在优化条件基础上进行传质性能和细胞生长特性实验，研究结果 表明ACA液芯微胶囊比ACA固芯微胶囊具有更好的传质特性，将ACA液芯微胶囊进行细胞包埋，液芯微胶囊较 固芯铹姐吏囊具有更高的细胞密度。 关键词：海藻酸盐一壳聚糖一海藻酸盐微胶囊；液芯；优化；响应面；Plackett—Burman法 中图分类号：O 636；R 318．08 文献标识码：A 文章编号：1000—6613(2010)O1—0124—06 Optimization of conditions for the preparation of alginate--chitosan—- alginate microcapsules with liquid core ZHUMinli，XUE Weiming，ZHA0Binran，LIUbo，XIEXiaohuan (Department ofChemical Technology，North West University，Xi’an 710069，Shaanxi，China) Abstract：The preparation conditions of alginate—chitosan—alginate(ACA)microcapsules with liquid corc and good mechanical strengtbwere optimized by response surface method．In the first optimization step，a Plackett·Burman design was used to evaluate the influence of related factors．The concentration and pH of chitosan solution have a positive effect to the membrane mechanical strength，while the pH of sodium citrate has a negativeeffect．The rest of other preparation conditions has no significant influence on the membrane mechanical strength．The path of steepest ascent was used to approach the optimal region of the optimized conditiOtiS subsequently~In the third step，the