化学生物组合法合成米格列醇及其表征.pdfVIP

学兔兔 化 工 进 展 2009年第28卷第3期 CHEMICAL INDUSTRY AND ENGINEERING PROGRESS 化学生物组合法合成米格列醇及其表征 王端好，孙 蓓，赵彭花，薛小波，张小里 (西北大学化工学院，陕西 西安 710069) 摘 要：以葡萄糖和乙醇胺为原料，催化加氢生成Ⅳ_羟乙基葡糖胺，葡萄糖转化率为89％。氧化葡萄糖酸杆菌Gouv2007 的静息细胞在通气下氧化Ⅳ-羟乙基葡糖胺脱氢生成米格列醇的前体物质6-脱氧一6-羟乙基氨基一 L厂呋喃山梨糖，再催 化加氢生成米格列醇，对从Ⅳ_羟乙基葡糖胺生成米格列醇的底物转化率为77．3％。采用熔程测定、红外光谱分析、质 谱分析对Ⅳ-羟乙基葡糖胺和米格列醇进行了表征，结果表明：所合成的产物分别被鉴定为Ⅳ．羟乙基葡糖胺和米格列醇。 关键词：氧化葡萄糖酸杆菌；N-羟乙基葡糖胺；微生物氧化；米格列醇 中图分类号：TQ463 文献标识码：A 文章编号：1000—6613(2009)03—0477—04 Synthesis and characterization of miglitol by combined bi0techn0l0gica1． chemical synthesis method WANG Duanhao，SUNBei，ZHA0 Penghua，XUEXiaobo，ZHANG Xiaoli (College ofChemical Engeedng，Northwest University，Xi’an 710069，Shaanxi，China) Abstract：Glucose and ethanolamines were catalytic hydrogenated to obtmn N(2·-hydroxyethy1)·- glucamine，with a transformation rate of 89％．N(2一hydroxyethy1)一glucamine was dehydrogenated to 6一r2一droxyethy1)amino一6一deoxy—a—L—sorbofiuanose the key intermediate of miglitol by the resting cells of Gluconoba~r oxydans Gouv2007 through aeration． 6一r2一Droxyethy1)amino一6一deoxy—a—L— sorbofiuanose was hydrogenated catalyticlly to miglito1．In the Vansformation of N(2一hydroxyethy1)一 glucamine to miglitol，the transformation rate was 77-3％．The samples of N(2一hydroxyethy1)一 glucamine and miglitol were characterized with infrared spectroscopy，melting point and MALDI—MS analysis．The results confirmed the samples of N(2一hydroxyethy1)一glucamine and miglito1． Key words：Gluconoba~r oxydans；N(2·-hydroxyethy1)··glucamine；microbial oxidation；miglitol 米格列醇 (miglito1)是一种新型0c一葡糖苷酶抑 了运用生