Cellular Autofluorescence following Ionizing Radiation 英文参考文献.docVIP

CellularAutofluorescencefollowingIonizingRadiation Do¨rtheSchaue*,JosephineA.Ratikan,KeisukeS.Iwamoto RadiationOncology,DavidGeffenSchoolofMedicineatUniversityofCaliforniaLosAngeles,LosAngeles,California,UnitedStatesofAmerica Abstract CellsoftenautofluoresceinresponsetoUVradiationexcitationandthiscanreflectcriticalaspectsofcellularmetabolism. Here we report that many different human and murine cell types respond to ionizing radiation with a striking rise in autofluorescence that is dependent on dose and time. There was a highly reproducible fluorescent shift at various wavelengths, which was mirrored by an equally reproducible rise in the vital intracellular metabolic co-factors FAD and NADH. It appears that mitochondria, metabolism and Ca2+ homeostasis are important for this to occur as cells without mitochondriaorcellsunabletoaltercalciumlevelsdidnotbehaveinthisway.Webelievetheseradiation-inducedchanges areofbiologicalimportanceandthatautofluorescencemayevenprovideuswithatooltomonitorradiationresponsesin theclinic. Citation: Schaue D, Ratikan JA, Iwamoto KS (2012) Cellular Autofluorescence following Ionizing Radiation. PLoS ONE 7(2): e32062. doi:10.1371/journal.pone. 0032062 Editor:BradleyStevenLaunikonis,UniversityofQueensland,Australia ReceivedNovember10,2011;AcceptedJanuary19,2012;PublishedFebruary22,2012 Copyright: ? 2012 Schaue et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:ThisstudywassupportedbytheDepartmentofDefensePC060403(DS),theDepartmentofDefenseBC094391(DS),andaNationalInstituteofAllergy andInfectiousDiseasesSeedgrant2U19AI-67769(DS).Thefundershadnoroleinstudydesign,datacollectionandanalysis,decisiontopublish,orpreparationof themanuscript. CompetingInterests:Theauthorshavedeclaredthatnocompetinginterestsexist. *E-mail:dschaue@ Introduction Methods Ethicsstatement