DRhoGEF2 Regulates Cellular Tension and Cell Pulsations in the Amnioserosa during Drosophila Dorsal Closure 英文参考文献.docVIP

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DRhoGEF2 Regulates Cellular Tension and Cell Pulsations in the Amnioserosa during Drosophila Dorsal Closure 英文参考文献.doc

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DRhoGEF2 Regulates Cellular Tension and Cell Pulsations in the Amnioserosa during Drosophila Dorsal Closure 英文参考文献

DRhoGEF2RegulatesCellularTensionandCellPulsations intheAmnioserosaduringDrosophilaDorsalClosure DulceAzevedo1,MarcoAntunes1,SorenPrag1,XiaoyanMa2,UdoHacker3,G.WayneBrodland4 ,M. ShaneHutson2,JeromeSolon5,AntonioJacinto1,6* 1Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Lisboa, Portugal, 2Vanderbilt Institute for Integrative Biosystems Research and Education,DepartmentofPhysicsandAstronomy,VanderbiltUniversity,Nashville,Tennessee,UnitedStatesofAmerica,3DepartmentofExperimentalMedicalScience, LundStrategicResearchCenterforStemCellBiologyandCellTherapy,LundUniversity,Lund,Sweden,4DepartmentofCivilandEnvironmentalEngineering,Universityof Waterloo,Waterloo,Ontario,Canada,5CRG-CentreforGenomicRegulation,Barcelona,Spain,6InstitutoGulbenkiandeCie?ncia,Oeiras,Portugal Abstract Coordinationofapicalconstrictioninepithelialsheetsisafundamentalprocessduringembryogenesis.Here,weshowthat DRhoGEF2 is a key regulator of apical pulsation and constriction of amnioserosal cells during Drosophila dorsal closure. AmnioserosalcellsmutantforDRhoGEF2exhibitaconsistentdecreaseinamnioserosapulsationswhereasoverexpressionof DRhoGEF2inthistissueleadstoanincreaseinthecontractiontimeofpulsations.Weprobedthephysicalpropertiesofthe amnioserosatoshowthattheaveragetensioninDRhoGEF2mutantcellsislowerthanwild-typeandthatoverexpressionof DRhoGEF2resultsinatissuethatismoresolid-likethanwild-type.WealsoobservethatintheDRhoGEF2overexpressing cellsthereisadramaticincreaseofapicalactomyosincoalescencethatcancontributetothegenerationofmorecontractile forces,leadingtoamnioserosalcellswithsmallerapicalsurfacethanwild-type.Conversely,inDRhoGEF2mutants,theapical actomyosin coalescence is impaired. These results identify DRhoGEF2 as an upstream regulator of the actomyosin contractilemachinerythatdrivesamnioserosacellspulsationsandapicalconstriction. Citation: Azevedo D, Antunes M, Prag S, Ma X, Hacker U, et al. (2011) DRhoGEF2 Regulates Cellular Tension and Cell Pulsations in