Fluorescence Correlation Spectroscopy Measurements of the Membrane Protein TetA in Escherichia coli Suggest Rapid Diffusion at Short Length Scales 英文参考文献.docVIP
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Fluorescence Correlation Spectroscopy Measurements of the Membrane Protein TetA in Escherichia coli Suggest Rapid Diffusion at Short Length Scales 英文参考文献
FluorescenceCorrelationSpectroscopyMeasurementsof
theMembraneProteinTetAinEscherichiacoliSuggest
RapidDiffusionatShortLengthScales
DavidChow1,LinGuo2,FengGai2,MarkGoulian1,3*
1Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America, 2Department of Chemistry, University of
Pennsylvania, Philadelphia, Pennsylvania, United States of America, 3Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania, United States of
America
Abstract
Structuralinhomogeneitiesinbiomembranescanleadtocomplexdiffusivebehaviorofmembraneproteinsthatdependon
thelengthortimescalesthatareprobed.Thiseffectiswellstudiedineukaryoticcells,buthasbeenexploredonlyrecently
in bacteria. Here we used fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spectroscopy
(FCS)tostudydiffusionofthemembraneproteinTetA-YFPinE.coli.Wefindthatthediffusionconstantdeterminedfrom
FRAPiscomparabletootherreportsofinnermembraneproteindiffusionconstantsinE.coli.However,FCS,whichprobes
diffusiononshorterlengthscales,givesavaluethatisalmosttwoordersofmagnitudehigherandiscomparabletolipid
diffusionconstants.TheseresultssuggestthereisapopulationofTetA-YFPmoleculesinthemembranethatmoverapidly
overshortlengthscales(,400nm)butmovesignificantlymoreslowlyoverthelongerlengthscalesprobedbyFRAP.
Citation:ChowD,GuoL,GaiF,GoulianM(2012)FluorescenceCorrelationSpectroscopyMeasurementsoftheMembraneProteinTetAinEscherichiacoliSuggest
RapidDiffusionatShortLengthScales.PLoSONE7(10):e48600.doi:10.1371/journal.pone.0048600
Editor:EricCascales,CentreNationaldelaRechercheScientifique,Aix-MarseilleUniversite′,France
ReceivedMay25,2012;AcceptedSeptember28,2012;PublishedOctober31,2012
Copyright: ? 2012 Chow et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited.
Funding:T
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