Global Gene Expression Analysis of Fission Yeast Mutants Impaired in Ser-2 Phosphorylation of the RNA Pol II Carboxy Terminal Domain 英文参考文献.docVIP

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Global Gene Expression Analysis of Fission Yeast Mutants Impaired in Ser-2 Phosphorylation of the RNA Pol II Carboxy Terminal Domain 英文参考文献.doc

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Global Gene Expression Analysis of Fission Yeast Mutants Impaired in Ser-2 Phosphorylation of the RNA Pol II Carboxy Terminal Domain 英文参考文献

GlobalGeneExpressionAnalysisofFissionYeastMutants ImpairedinSer-2PhosphorylationoftheRNAPolII CarboxyTerminalDomain RezaSaberianfar,StephenCunningham-Dunlop,JimKaragiannis* DepartmentofBiology,UniversityofWesternOntario,London,Ontario,Canada Abstract In Schizosaccharomyces pombe the nuclear-localized Lsk1p-Lsc1p cyclin dependent kinase complex promotes Ser-2 phosphorylationoftheheptadrepeatsfoundwithintheRNApolIIcarboxyterminaldomain(CTD).Here,wefirstprovide evidencesupportingtheexistenceofathirdpreviouslyuncharacterizedSer-2CTDkinasesubunit,Lsg1p.Asexpectedfora component of the complex, Lsg1p localizes to the nucleus, promotes Ser-2 phosphorylation of the CTD, and physically interactswithbothLsk1pandLsc1pinvivo.Interestingly,wealsodemonstratethatlsg1Dmutants–justlikelsk1Dandlsc1D strains–arecompromisedintheirabilitytofaithfullyandreliablycompletecytokinesis.Next,toaddresswhetherkinase mediatedalterationsinCTDphosphorylationmightselectivelyaltertheexpressionofgeneswithrolesincytokinesisand/or thecytoskeleton,globalgeneexpressionprofileswereanalyzed.MutantsimpairedinSer-2phosphorylationdisplaylittle changewithrespecttotheleveloftranscriptionofmostgenes.However,genesaffectingcytokinesis–includingtheactin interactingproteingene,aip1–aswellasgeneswithrolesinmeiosis,areincludedinasmallsubsetthataredifferentially regulated.Significantly,geneticanalysisoflsk1Daip1DdoublemutantsisconsistentwithLsk1pandAip1pactinginalinear pathwaywithrespecttotheregulationofcytokinesis. Citation:SaberianfarR,Cunningham-DunlopS,KaragiannisJ(2011)GlobalGeneExpressionAnalysisofFissionYeastMutantsImpairedinSer-2Phosphorylation oftheRNAPolIICarboxyTerminalDomain.PLoSONE6(9):e24694.doi:10.1371/journal.pone.0024694 Editor:MichaelPolymenis,TexasAMUniversity,UnitedStatesofAmerica ReceivedJuly27,2011;AcceptedAugust15,2011;PublishedSeptember12,2011 Copyright: ?2011Saberianfaretal.Thisisanopen-access articledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermits unrestricteduse