Molecular Characterization of the Measles Virus Genotypes in JiLin Province, China 英文参考文献.docVIP

MolecularCharacterizationoftheMeaslesVirus GenotypesinJiLinProvince,China ChengguoWei1.,JingweiShi1.,BinLiu3,YueShi1,JingtongZheng1,GuangyuXu1,JinshuMa1, GuoqingWang1,2*,FanLi1,2 * 1Department of Pathogeny Biology, Norman Bethune Medical College of Jilin University, Changchun,Jilin, China, 2Key Laboratory of Zoonosis Research,Ministry of Education,JilinUniversity,,Changchun,Jilin,China,3TheFirstBethuneHospitalofJilinUniversity,Changchun,Jilin,China Abstract Measles remains a severe global health threat, and nearly 30 million new cases are reported annually. Although many studies have analyzed measles viruses (MV) at the epidemiologic and phylogenetic levels, no study has yet to integrate thesetwotypesofdata.Tothisend,weisolated16wild-typeMVstrainsChina’sJilinprovince.TheMVgenotypeH1wasthe mostprevalentstrain.Aftersequencingthenucleoprotein(N)genesofthesestrains,amaximumcladecredibilitytreewas constructedbytheBayesianMarkovChainMonteCarlomethodusing450MVstrainsfromGenBankwithepidemiological information.TheMVNgeneevolutionratewas1.127E-3.Analysisofthetimeofthemostrecentcommonancestor(TMRCA) for genotypes A/B/C/G/H revealed that genotypes D and B had the largest and smallest TMRCA (45.86 and 26.63, respectively).ThehighestlevelofgeneticdiversityfortheMVNgeneoccurredaroundtheyear2000.Hereinthisstudy,we uncovered the MV genotypes circulating in China’s Jilin Province and estimated the epidemiologic and phylogenetic relationshipforthesixdifferentgenotypesofMV. Citation:WeiC,ShiJ,LiuB,ShiY,ZhengJ,etal.(2012)MolecularCharacterizationoftheMeaslesVirusGenotypesinJiLinProvince,China.PLoSONE7(10): e46011.doi:10.1371/journal.pone.0046011 Editor:CecilioLo′pez-Gal?′ndez,CentroNacionaldeMicrobiolog?′a-InstitutodeSaludCarlosIII,Spain ReceivedMay18,2012;AcceptedAugust23,2012;PublishedOctober8,2012 Copyright:?2012Weietal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,whichpermitsunrestricted use,distribution,andreproductioninanymedium,provid