Novel Processed Form of Syndecan-1 Shed from SCC-9 Cells Plays a Role in Cell Migration 英文参考文献.docVIP
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Novel Processed Form of Syndecan-1 Shed from SCC-9 Cells Plays a Role in Cell Migration 英文参考文献
NovelProcessedFormofSyndecan-1ShedfromSCC-9
CellsPlaysaRoleinCellMigration
AnnelizeZ.B.Araga?o1.,Mar?′liaBelloni1.,FernandoM.Simabuco1,MarianaR.Zanetti1,SamiYokoo1,
Rome?niaR.Domingues1,RebecaKawahara1,BiancaA.Pauletti1,AndersonGonc?alves1,
MichelleAgostini2,EdgardGraner2,RicardoD.Coletta2,JayW.Fox3,AdrianaF.PaesLeme1*
1MassSpectrometryLaboratory,BrazilianBiosciencesNationalLaboratory–CNPEM,Campinas,Brazil,2PiracicabaSchoolofDentistry,UniversityofCampinas(UNICAMP),
Piracicaba,Brazil,3SchoolofMedicine,UniversityofVirginia,Charlottesville,Virginia,UnitedStatesofAmerica
Abstract
Theextracellularmilieuiscomprisedinpartbyproductsofcellularsecretionandcellsurfaceshedding.Thepresenceof
such molecules of the sheddome and secretome in the context of the extracellular milieu may have important clinical
implications.Incancertheyhavebeenhypothesizedtoplayaroleintumorgrowthandmetastasis.Theobjectiveofthis
studywastoevaluatewhetherthesheddome/secretomefromtwocelllinescouldbecorrelatedwiththeirpotentialfor
tumor development. Two epithelial cell lines, HaCaT and SCC-9, were chosen based on their differing abilities to form
tumors in animal models of tumorigenesis. These cell lines when stimulated with phorbol-ester (PMA) showed different
characteristicsasassessedbycellmigration,adhesionandhighergelatinaseactivity.Proteomicanalysisofthemediafrom
thesetreatedcellsidentifiedinteresting,functionallyrelevantdifferencesintheirsheddome/secretome.Amongtheshed
proteins, solublesyndecan-1 wasfoundonlyinmedia fromstimulated tumorigenic cells (SCC-9)anditsfragments were
observedinhigheramountinthestimulatedtumorigeniccellsthanstimulatednon-tumorigeniccells(HaCaT).Theincrease
insolublesyndecan-1wasassociatedwithadecreaseinmembrane-boundsyndecan-1ofSCC-9cellsafterPMAstimuli.To
supportafunctionalroleforsolublesyndecan-1fragmentswedemonstratedthatthesyntheticsyndecan-1peptidewas
able to induce cell migration in both cell lines. Taken together, these results suggested that PMA stimulation alte
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