菌株GL的鉴定及其与科罗拉多冷杉合成.PDF

GL 1 1 2 *1 王丽华 张金冬 李志丹 张金凤 ( 1 2) : 从形态学和分子生物学两方面对菌株 GL做了鉴定, 初步 为它是裂褶菌属的 一种进而将菌株 GL 与 科罗拉多冷杉进行合成实验, 通过感染率生长指标测定以及石蜡切片观察, 得出菌株 GL 是科罗拉多冷杉外生菌 根菌的结论 : 裂褶菌; 科罗拉多冷杉; 鉴定; 合成 Identification o f Strain GL and the F orm a tion Research B etw een GL andA bies concolor MWANG L-i hua, ZHANG Jin-Dong, LI Zh-i dan, ZHANG Jin-F ng A bstract: Strain GL w as prmi arily id ntifi d as Sch izop hyllum bas d on th prop rti s ofm orphology and m ol cular b iology. Th n th form ation r s arch b tw n strain GL andA bies concolor was stud i d. By th inocu lating rat , grow th ind x s and paraffin s ction, it w as conclud d that th strain GL w as ctom ycorrh iza ofA b ies concolor K ey w ord s: Sch izop hy llum; A b ies concolor; Id n tification; Formation F irst author. s address: K y Laboratory of G n tic and Br d ing in For st Tr s and O rnam n tal Plants, M in istry of Edu- cation, Coll g of B iosci nc and B iot chnology, B ijing For stry Univ rsity, 100083, B ijing, China [ 2] (A bies concolor ), OLYM PAS BX51 , , , 1. 2. 2 分子生物学鉴定 , 1. 2. 2. 1 真菌基因组DNA 提取 [ 1] [ 3] , , DNA M oll r , , , : 0. 5~ 1g, GL, ; 3 mL 65e DNA , , ( 100 mM Tris-HC,l 20mM EDTA, 115 M NaC,l 2% GL , CTAB, 4% PVP, 2% , pH 8. 0), 65e 30 m in