Pro-Inflammatory Cytokines, IFNγ and TNFα, Influence Immune Properties of Human Bone Marrow and Wharton Jelly Mesenchymal Stem Cells Differentially 英文参考文献.docVIP
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Pro-Inflammatory Cytokines, IFNγ and TNFα, Influence Immune Properties of Human Bone Marrow and Wharton Jelly Mesenchymal Stem Cells Differentially 英文参考文献
Pro-InflammatoryCytokines,IFNcandTNFa ,Influence
ImmunePropertiesofHumanBoneMarrowandWharton
JellyMesenchymalStemCellsDifferentially
S.JyothiPrasanna*,DivyaGopalakrishnan.,ShilpaRaniShankar.,AnoopBabuVasandan.
ManipalInstituteofRegenerativeMedicine,ConstituentInstituteofManipalUniversity,Bangalore,Karnataka,India
Abstract
Background: Wharton’s jelly derived stem cells (WJMSCs) are gaining attention as a possible clinical alternative to bone
marrow derived mesenchymal stem cells (BMMSCs) owing to better accessibility, higher expansion potential and low
immunogenicity. Usage of allogenic mesenchymal stem cells (MSC) could be permissible in vivo only if they retain their
immune properties in an inflammatory setting. Thus the focus of this study is to understand and compare the immune
propertiesofBMMSCsandWJMSCsprimedwithkeypro-inflammatorycytokines,Interferon-c(IFNc)andTumorNecrosis
Factor-a(TNFa).
Methodology/PrincipalFindings:InitiallytheeffectofprimingonMSCmediatedsuppressionofalloantigenandmitogen
inducedlymphoproliferationwasevaluatedinvitro.TreatmentwithIFNcorTNFa,didnotablatetheimmune-suppression
caused by both the MSCs. Extent of immune-suppression was more with WJMSCs than BMMSCs in both the cases.
Surprisingly, priming BMMSCs enhanced suppression of mitogen driven lymphoproliferation only; whereas IFNc primed
WJMSCswerebettersuppressorsofMLRs.Further,kineticanalysisofcytokineprofilesinco-culturesofprimed/unprimed
MSCsandPhytohematoagglutinin(PHA)activatedlymphocyteswasevaluated.Resultsindicatedadecreaseinlevelsofpro-
inflammatorycytokines.Interestingly,achangeinkineticsandthresholdsofInterleukin-2(IL-2)secretionwasobservedonly
withBMMSCs.AnalysisofactivationmarkersonPHA-stimulatedlymphocytesindicateddifferentexpressionpatternsinco-
culturesofprimed/unprimedWJMSCsandBMMSCs.Strikingly,co-culturewithWJMSCsresultedinanearlyactivationofa
negativeco-stimulatorymolecule,CTLA4,whichwasnotevidentwithBMMSCs.Ascreenforimmunesuppressivefactorsin
primed/unprimed WJMSCs
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