Production and downstream processing of (1→3)-β-D-glucan from mutant strain of Agrobacterium sp. ATCC 31750 英文参考文献.docVIP

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Production and downstream processing of (1→3)-β-D-glucan from mutant strain of Agrobacterium sp. ATCC 31750 英文参考文献.doc

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Production and downstream processing of (1→3)-β-D-glucan from mutant strain of Agrobacterium sp. ATCC 31750 英文参考文献

Kalyanasundarametal.AMBExpress2012,2:31 /content/2/1/31 ORIGINAL ARTICLE OpenAccess Productionanddownstreamprocessingof (1!3)-β-D-glucanfrommutantstrainof Agrobacteriumsp.ATCC31750 GayathiriTKalyanasundaram,MukeshDobleandSathyanarayanaNGummadi* Abstract WeisolatedamutantthatproducedhigherlevelsofcurdlanthanthewildstrainAgrobacteriumsp.ATCC31750by chemicalmutagenesisusingN-methyl-N-nitro-nitrosoguanidine.Themutantstrainproduced66g/Lofcurdlanin 120hwithayieldof(0.88)while,thewildstrainproduced41g/Lin120hwithayieldof(0.62)inastirred bioreactor.ThemutantcouldnotproducecurdlanwhenthepHwasshiftedfrom7.0to5.5afternitrogen depletionasfollowedforwildstrain.Incontrast,pHoptimumforcellgrowthandcurdlanproductionformutant wasfoundtobe7.0.WeoptimizedthedownstreamprocessingofcurdlanbyvaryingdifferentvolumesofNaOH andHClforextractionandprecipitationofcurdlan.Themolecularweightofthepurifiedcurdlanfromthewildand mutantstrainwas6.6×105Daand5.8×105Darespectively.Themonosaccharideanalysesconfirmthatcurdlan frombothwildandmutantstraincontainsonlyglucoseunits.FromtheNMRandFTIRdata,ithasbeenconfirmed thatcurdlanwasexclusivelycomposedofβ(1!3)-D-glucanresidues. Keywords:Curdlan,Agrobacteriumsp.ATCC31750,Chemicalmutagenesis,Twostageculture,Downstream processing,Characterization Introduction theconcrete(Leeetal.1997Kimetal.2000).Itisalso Curdlanisahighmolecularweight,waterinsoluble(alkali used as an immobilization support as it can covalently soluble) extracellular polysaccharide composed only of link available amino, hydroxyl and sulfhydryl groups of β-(1!3) glucose residues (Lee et al. 1999). It acts as a enzymes(Leeetal.1999).Curdlansulphateisdeveloped structuralmacromoleculeinthecellwallofyeast,mush- as an antiviral agent against human immunodeficiency roomsandotherhigherplants(KoandLin2004).Curdlan virus infections (Lee and Park 2001 Zhang et al. isasecondarymetabolitesynthesizedbyAlcaligenesfaeca- 2012). Curdlan stimulates nuclear factor kappa-B in lis var. myxo