Prototypes of Newly Conceived Inorganic and Biological Sensors for Health and Environmental Applications 英文参考文献.docVIP

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Prototypes of Newly Conceived Inorganic and Biological Sensors for Health and Environmental Applications 英文参考文献.doc

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Prototypes of Newly Conceived Inorganic and Biological Sensors for Health and Environmental Applications 英文参考文献

Sensors 2012, 12, 17112-17127; doi:10.3390/s121217112 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Prototypes of Newly Conceived Inorganic and Biological Sensors for Health and Environmental Applications Claudio Nicolini 1,2,*, Manuela Adami 3, Marco Sartore 3, Nicola Luigi Bragazzi 1, Valter Bavastrello 1, Rosanna Spera 1 and Eugenia Pechkova 1,2 1 Laboratories of Biophysics and Nanobiotechnology, Department of Experimental Medicine (DIMES), University of Genoa, Genoa 16132, Italy; E-Mails: nicola@genethics.ethicsoft.it (N.L.B.); vbavastrello@ (V.B.); rspera@ibf.unige.it (R.S.); epechkova@ibf.unige.it (E.P.) 2 Nanoworld Institute Fondazione EL.B.A. Nicolini, Bergamo 24100, Italy 3 Elbatech Srl, Marciana, Marciana 57030, Italy; E-Mails: adami@ (M.A.); sartore@ (M.S.) * Author to whom correspondence should be addressed; E-Mail: cnicolini@ibf.unige.it; Tel.: +39-010-353-38217; Fax: +39-010-353-38215. Received: 8 October 2012; in revised form: 7 December 2012 / Accepted: 11 December 2012 / Published: 12 December 2012 Abstract: This paper describes the optimal implementation of three newly conceived sensors for both health and environmental applications, utilizing a wide range of detection methods and complex nanocomposites. The first one is inorganic and based on matrices of calcium oxide, the second is based on protein arrays and a third one is based on Langmuir-Blodgett laccase multi-layers. Special attention was paid to detecting substances significant to the environment (such as carbon dioxide) and medicine (drug administration, cancer diagnosis and prognosis) by means of amperometric, quartz crystal microbalance with frequency (QCM_F) and quartz crystal microbalance with dissipation monitoring (QCM_D) technologies. The resulting three implemented nanosensors are described here along with proofs of principle and their corresponding applications. Keywords: QCM_D; protein-protein in