Rapid and Sensitive MicroRNA Detection with Laminar Flow-Assisted Dendritic Amplification on Power-Free Microfluidic Chip 英文参考文献.docVIP

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Rapid and Sensitive MicroRNA Detection with Laminar Flow-Assisted Dendritic Amplification on Power-Free Microfluidic Chip 英文参考文献.doc

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Rapid and Sensitive MicroRNA Detection with Laminar Flow-Assisted Dendritic Amplification on Power-Free Microfluidic Chip 英文参考文献

RapidandSensitiveMicroRNADetectionwithLaminar Flow-AssistedDendriticAmplificationonPower-Free MicrofluidicChip HideyukiArata¤,HiroshiKomatsu,KazuoHosokawa*,MizuoMaeda BioengineeringLaboratory,RIKEN,Wako,Japan Abstract DetectionofmicroRNAs,smallnoncodingsingle-strandedRNAs,isoneofthekeytopicsinthenewgenerationofcancer researchbecausecancerinthehumanbodycanbedetectedorevenclassifiedbymicroRNAdetection.Thisreportshows rapid and sensitive microRNA detection using a power-free microfluidic device, which is driven by degassed poly(dimethylsiloxane), thus eliminating the need for an external power supply. MicroRNA is detected by sandwich hybridization,andthesignalisamplifiedbylaminarflow-assisteddendriticamplification.Thismethodallowsustodetect microRNAofspecificsequencesatalimitofdetectionof0.5pMfroma0.5mLsamplesolutionwithadetectiontimeof 20min.Togetherwiththeadvantagesofself-relianceofthisdevice,thismethodmightcontributesubstantiallytofuture point-of-careearly-stagecancerdiagnosis. Citation: ArataH, KomatsuH, Hosokawa K,MaedaM(2012)Rapid andSensitive MicroRNA DetectionwithLaminarFlow-AssistedDendriticAmplificationon Power-FreeMicrofluidicChip.PLoSONE7(11):e48329.doi:10.1371/journal.pone.0048329 Editor:Wei-ChunChin,UniversityofCalifornia,Merced,UnitedStatesofAmerica ReceivedJune19,2012;AcceptedSeptember24,2012;PublishedNovember7,2012 Copyright: ? 2012 Arata etal. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding: This work was supported by KAKENHI and the Funding Program for World-Leading Innovative RD on Science and Technology. The fundershadnoroleinstudydesign,datacollectionandanalysis,decisiontopublish,orpreparationofthemanuscript. CompetingInterests:Theauthorshavedeclaredthatnocompetinginterestsexist. *E-mail:k-hoso@riken.go.jp ¤Currentaddress:ExploratoryResearchforAdvanced