Regulation of Mariner Transposition The Peculiar Case of Mos1 英文参考文献.docVIP

RegulationofMarinerTransposition:ThePeculiarCaseof Mos1 Je′ro?meJaillet1,MurielleGenty1,JeanneCambefort1,Jacques-DericRouault2,3,CorinneAuge′-Gouillou1* 1InnovationMole′culaireThe′rapeutique,EA6306–Universite′ Franc?ois Rabelais,ParcGrandmont,Tours,France,2LaboratoireEvolution,Ge′nomesetSpe′ciation–CNRS UPR9034,Gif-sur-Yvette,France,3Universite′ Paris-Sud11,Orsay,France Abstract Background: Mariner elements represent the most successful family of autonomous DNA transposons, being present in variousplantandanimalgenomes,includinghumans.Theintroductionandco-evolutionofmarinerswithinhostgenomes imply astrict regulation of thetransposon activity.Biochemical dataaccumulated during thepast decade haveled toa convergentpictureofthetranspositioncycleofmarinerelements,suggestingthatmarinertranspositiondoesnotrelyon host-specific factors. This model does not account for differences of transposition efficiency in human cells between mariners. We thus wondered whether apparent similarities in transposition cycle could hide differences in the intrinsic parametersthatcontrolmarinertransposition. Principal Findings: We find that Mos1 transposase concentrations in excess to the Mos1 ends prevent the paired-end complex assembly. However, we observe that Mos1 transposition is not impaired by transposase high concentration, dismissing the idea that transposase over production plays an obligatory role in the down-regulation of mariner transposition.Ourmainfindingisthatthepaired-endcomplexisformedinacooperativeway,regardlessofthetransposase concentration.WealsoshowthatanelementframedbytwoidenticalITRs(InvertedTerminalRepeats)ismoreefficientin driving transposition than an element framed by two different ITRs (i.e. the natural Mos1 copy), the latter being more sensitivetotransposaseconcentrationvariations.Finally,weshowthatthecurrentMos1ITRscorrespondtotheancestral ones. Conclusions: We provide new insights on intrinsic properties supporting the self-regulation of the Mos1 elemen