雌激素缺乏型人骨髓间充质干细胞肾虚表型的验证.docVIP

雌激素缺乏型人骨髓间充质干细胞肾虚表型的验证 魏秋实1，2，何 伟1，2，陈镇秋1，陈 达2，洪郭驹2，陈建发1，杨 鹏2(1广州中医药大学第一附属医院骨科，广东省广州市 510407；2广州中医药大学国家重点学科中医骨伤科学实验室，广东省广州市 510405) 引用本文：魏秋实，何伟，陈镇秋，陈达，洪郭驹，陈建发，杨鹏. 雌激素缺乏型人骨髓间充质干细胞肾虚表型的验证[J].中国组织工程研究，2017，21():669-675. DOI: 10.3969/j.issn.2095-4344.2017.05.003 ORCID: 0000-0002-1274-9997(魏秋实) 文章快速阅读： 文题释义： 环磷酸腺苷(cAMP)A preliminary proof of kidney-deficiency phenotype in human bone marrow mesenchymal stem cells with estrogen deficiency Wei Qiu-shi1, 2, He Wei1, 2, Chen Zhen-qiu1, Chen Da2, Hong Guo-ju2, Chen Jian-fa1, Yang Peng2 (1Department of Orthopedics Surgery, First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510407, Guangdong Province, China; 2Key Laboratory of Osteology and Traumatology of TCM, Guangzhou University of Chinese Medicine, Guangzhou 510405, Guangdong Province, China) Abstract BACKGROUND: As the presence of decreased proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs) results from estrogen deficiency-induced postmenopausal osteoporosis, it is of significance to study the relevant pathogenesis and treatment strategies from the perspective of estrogen and its receptors. OBJECTIVE: To observe the characteristics of kidney-deficiency phenotype in estrogen deficiency-induced BMSCs. METHODS: Passage 3 BMSCs were cultured in the osteogenic induction medium. The cells were divided into five groups, and cultured with different concentrations (0, 10-10, 10-9, 10-8, 10-7 mol/L) of letrozole. BMSCs with no osteogenic induction were used as blank controls. The expression levels of AROM were detected at mRNA and protein levels by real-time PCR and western blot methods. Cell proliferation of BMSCs was detected using MTT assay. Alkaline phosphatase activity assay was used to detect BMSCs osteogenic ability. ELISA was used to detect estradiol levels in cell supernatants. The expression of cAMP mRNA was detected by real-time PCR method. RESULTS AND CONCLUSION: 10-8 mol/L Letrozole exhibited the