Crystal Structure of ATVORF273, a New Fold for a Thermo- and Acido-Stable Protein from the Acidianus Two-Tailed Virus 英文参考文献.docVIP

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Crystal Structure of ATVORF273, a New Fold for a Thermo- and Acido-Stable Protein from the Acidianus Two-Tailed Virus 英文参考文献.doc

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Crystal Structure of ATVORF273, a New Fold for a Thermo- and Acido-Stable Protein from the Acidianus Two-Tailed Virus 英文参考文献

CrystalStructureofATVORF273,aNewFoldforaThermo- andAcido-StableProteinfromtheAcidianusTwo-Tailed Virus CatarinaFelisberto-Rodrigues1,Ste′phanieBlangy1,AdelineGoulet1¤,GisleVestergaard2, ChristianCambillau1,RogerA.Garrett2,MiguelOrtiz-Lombard?′a1* 1CNRS, Aix-Marseille Universite′, AFMB, UMR 7257, Campus de Luminy, Marseille, France, 2Archaea Centre, Department of Biology, University of Copenhagen, Copenhagen,Denmark Abstract Acidianustwo-tailedvirus(ATV)infectscrenarchaeaofthegenusAcidianuslivinginterrestrialthermalspringsatextremely high temperatures and low pH. ATV is a member of the Bicaudaviridae virus family and undergoes extra-cellular developmentoftwotails,aprocessthatisuniqueintheviralworld.Tounderstandthisintriguingphenomenon,wehave undertaken structural studies of ATV virion proteins and here we present the crystal structure of one of these proteins, ATVORF273.ATVORF273 formstetramersinsolutionandamolecularenvelopeisprovidedforthetetramer,computedfrom small-angle X-ray scattering (SAXS) data. The crystal structure has properties typical of hyperthermostable proteins, includingarelativelyhigh numberofsaltbridges.However,theproteinalsoexhibitsflexible loopsandsurface pockets. Remarkably, ATVORF273 displays a new azb protein fold, consistent with the absence of homologues of this protein in publicsequencedatabases. Citation:Felisberto-RodriguesC,BlangyS,GouletA,VestergaardG,CambillauC,etal.(2012)CrystalStructureofATVORF273,aNewFoldforaThermo-andAcido- StableProteinfromtheAcidianusTwo-TailedVirus.PLoSONE7(10):e45847.doi:10.1371/journal.pone.0045847 Editor:NarayanaswamySrinivasan,IndianInstituteofScience,India ReceivedMay24,2012;AcceptedAugust23,2012;PublishedOctober8,2012 Copyright:?2012Felisberto-Rodriguesetal.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense,which permitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalauthorandsourcearecredited. Funding:C.F.-R.wasfundedbyadoctoralfello