Single-prolonged stress induces apoptosis in dorsal raphe nucleus in the rat model of posttraumatic stress disorder 英文参考文献.docVIP

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Single-prolonged stress induces apoptosis in dorsal raphe nucleus in the rat model of posttraumatic stress disorder 英文参考文献.doc

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Single-prolonged stress induces apoptosis in dorsal raphe nucleus in the rat model of posttraumatic stress disorder 英文参考文献

Liuetal.BMCPsychiatry2012,12:211 /1471-244X/12/211 RESEARCH ARTICLE OpenAccess Single-prolongedstressinducesapoptosisin dorsalraphenucleusintheratmodelof posttraumaticstressdisorder DongjuanLiu1,2,BingXiao1,FangHan2,EnhuaWang3andYuxiuShi1,2,3* Abstract Introduction:Post-traumaticstressdisorder(PTSD)isananxietydisorderthatdevelopsafterexposuretoa life-threateningtraumaticexperience.Meta-analysesofthebrainstemshowedthatmidsagittalareaoftheponswas significantlyreducedinpatientswithPTSD,suggestingapotentialapoptosisindorsalraphenucleusafter single-prolongedstress(SPS).TheaimofthisstudyistoinvestigatewhetherSPSinducesapoptosisindorsalraphe nucleusinPTSDrats,whichmaybeapossiblemechanismofreducedvolumeofponsanddensityofgraymatter. Methods:Inthisstudy,ratswererandomlydividedinto1d,7dand14dgroupsafterSPSalongwiththecontrol group.TheapoptosisratewasdeterminedusingannexinV-FITC/PIdouble-labeledflowcytometry(FCM).Levelsof Cytochromec(Cyt-C)wasexaminedbyWesternblotting.ExpressionofCyt-Conmitochondriainthedorsalraphe nucleusneuronwasdeterminedbyenzymohistochemistryundertransmissionelectronmicroscopy(TEM).The changeofthiaminemonophosphatase(TMP)levelswasassessedbyenzymohistochemistryunderlightmicroscope andTEM.Morphologicalchangesoftheultrastructureofthedorsalraphenucleusneuronweredeterminedby TEM. Results:ApoptoticmorphologicalalterationswereobservedindorsalraphenucleusneuronforallSPS-stimulate groupsofrats.TheapoptosisratesweresignificantlyincreasedindorsalraphenucleusneuronofSPSrats,along withincreasedreleaseofcytochromecfromthemitochondriaintothecytoplasm,increasedexpressionofCyt-C andTMPlevelsinthecytoplasm,whichreachedtothepeakofincrease7daysofSPS. Conclusions:TheresultsindicatethatSPSinducedCyt-Creleasedfrommitochondriaintocytosolandapoptosisin dorsalraphenucleusneuronofrats.IncreasedTMPincytoplasmfacilitatedtheclearanceofapoptoticcells.We proposethatthispresentsoneofthemechanismsthatleadtoreducedvolumeofponsandgraymatterassociated withPTSD. Keywords:Posttraumaticstressd