Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka′s reaction and Quartz Crystal Microbalance 英文参考文献.docVIP

Sensors 2009, 9, 1355-1369; doi:10.3390/OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka′s reaction and Quartz Crystal Microbalance Dalibor Huska 1, Ivo Fabrik 1, Jiri Baloun 1, Vojtech Adam 1,2, Michal Masarik 3, Jaromir Hubalek 4, Anna Vasku 3, Libuse Trnkova 5, Ales Horna 6, Ladislav Zeman 2 and Rene Kizek 1,* 1 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic 2 Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-613 00 Brno, Czech Republic 3 Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic 4 Department of Microelectronics, Faculty of Electrical Engineering and Communication, Brno University of Technology, Udolni 53, CZ-602 00 Brno, Czech Republic 5 Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic 6 Tomas Bata University, T.G. Masaryka 275, CZ-762 72 Zlin, Czech Republic * Author to whom correspondence should be addressed; E-Mail:kizek@sci.muni.cz Received: 8 October 2008; in revised form: 14 February 2009 / Accepted: 17 February 2009 / Published: 26 February 2009 Abstract: Treatment strategies for tumour diseases are progressively focusing on personalization of medicine. However, this focus requires methods revealing the early general biological mechanisms, including the formation anti-cancer drugs’ resistance. The low molecular mass protein metallothionein is thought to be the crucial for the formation of resistance in tumour treatment based on the platinum-cytostatics. The interactions between metallothionein (MT) and cisplatin were determined by the adsorptive transfer s