The brain slice method for studying drug distribution in the CNS 英文参考文献.docVIP

Loryanetal.FluidsandBarriersoftheCNS2013,10:6 /content/10/1/6 FLUIDS AND BARRIERS OF THE CNS STUDY PROTOCOL OpenAccess Thebrainslicemethodforstudyingdrug distributionintheCNS IrenaLoryan1,MarkusFridén2andMargaretaHammarlund-Udenaes1* Abstract Thehigh-throughputbrainslicemethodisapreciseandrobusttechniqueforestimatingtheoveralluptakeofdrugs intobraintissuethroughdeterminationoftheunboundvolumeofdistributioninthebrain(Vu,brain;ml·gbrain-1). Vu,brain describestherelationshipbetweenthetotaldrugconcentrationinthebrainandtheconcentrationof unbounddruginthebraininterstitialfluid,regardlessofblood–b