Thermoresponsive Magnetic Nano-Biosensors for Rapid Measurements of Inorganic Arsenic and Cadmium 英文参考文献.docVIP

Sensors 2012, 12, 14041-14052; doi:10.3390/s121014041 OPEN ACCESS sensors ISSN 1424-8220 /journal/sensors Article Thermoresponsive Magnetic Nano-Biosensors for Rapid Measurements of Inorganic Arsenic and Cadmium Mohammad Shohel Rana Siddiki 1, Shun Shimoaoki 2, Shunsaku Ueda 1,2 and Isamu Maeda 1,2, * 1 United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu 183-8509, Japan; E-Mails: msrsiddiki@ (M.S.R.S.); uedashun@cc.utsunomiya-u.ac.jp (S.U.) 2 Faculty of Agriculture, Utsunomiya University, 350 Minemachi, Utsunomiya 321-8505, Japan; E-Mail: metal_gear1964@yahoo.co.jp * Author to whom correspondence should be addressed; E-Mail: i-maeda@cc.utsunomiya-u.ac.jp; Tel./Fax: +81-28-649-5477. Received: 9 July 2012; in revised form: 3 September 2012 / Accepted: 8 October 2012 / Published: 18 October 2012 Abstract: Green fluorescent protein-tagged sensor proteins, ArsR-GFP and CadC-GFP, have been produced as biosensors for simple and low-cost quantification of As(III) or Cd(II). In this study, the sensor protein-promoter DNA complexes were reconstructed on the surfaces of magnetic particles of different sizes. After the surface modification all the particles could be attracted by magnets, and released different amounts of GFP-tagged protein, according to the metal concentrations within 5 min, which caused significant increases in fluorescence. A detection limit of 1 μg/L for As(III) and Cd(II) in purified water was obtained only with the nanoparticles exhibiting enough magnetization after heat treatment for 1 min. Therefore, thermoresponsive magnetic nano-biosensors offer great advantages of rapidity and sensitivity for the measurement of the toxic metals in drinking water. Keywords: magnetic nanoparticles; heavy metal; biosensor; ArsR; CadC; GFP 1. Introduction Magnetic separation and nanotechnology have attracted much attention, and could be applie