毕业论文终版.docVIP

分类号 ： 学校代码 ： 10426 密级 ： 学号 ： 硕 士 学 位 论 文 MASTER DEGREE THESIS DNA循环放大生物传感方法的研究 作 者 ： 指导教师 ： 学科专业 ： 化 学 专业代码 ： 07030 研究方向 ： 2014 年 月 日 基于表面增强拉曼技术的DNA循环放大生物传感方法的研究 摘 要 本文主要做了以下几个方面的工作：一是基于表面增强拉曼光谱（surface enhanced Raman scattering spectrum, SERS）检测技术构建了一种新型的DNA循环放大方法，利用DNA酶和目标分子结合后独特的共反应催化剪切活性(3σ)。利用引发链的再循环利用，固定在磁珠上的发卡探针被不断的打开，进而大量的生物条码被固定到了磁珠上，实现了高效的源头信号放大，并通过磁性分离和洗涤，除去过量的纳米金生物条码，降低了检测体系的背景值，从而实现了的高灵敏度检测可用于实际样品细胞匀浆中的-组氨酸的测定。 BIOSENSING METHOD RESEARCH WITH DNA-RELATED CYCLES AMPLIFICATION USING SERS DETECTION TECHNOLOGY ABSTRACT In this study, a series of SERS signal amplification biosensing methods were developed with high sensitivity, easy operation and good reproducibility. First, a novel SERS method was designed to detect L-histidine based on DNA amplification technique. The DNAzyme as a molecular recognition module will trigger circular hairpin assisted exponential amplification reaction simultaneously. Second, two kinds of differently modified bio-barcodes were prepared to detect the corresponding two kinds of genes based on autonomous target-displacement polymerization reaction. On this basis, a difunctional aptamer probe was introduced into the system to detect ATP and lysozyme simultaneously. These SERS biosensing method provides a universal method for quantitative analysis of genes, cells and also proteins, etc, and supplies valuable information for biomedical research. 1. A novel SERS method based on DNAzyme which can specifically recognize L-histidine is demonstrated in this assay to detect L-histidine. It was confirmed that the unmodi?ed DNAzyme had characteristic of endonuclease as long as it combined with the target. In the DNA recycling amplification reaction, a large number of bio-barcodes were indirectly captured on the magnetic beads as the hairpin probes immobilized on the magnetic beads were constantly opened. The problem