原创力文档A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf.docVIP
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A Novel mRNA Level Subtraction Method for Quick Identification of Target-Orientated Uniquely Expressed Genes Between Peanut Immature Pod and Leaf
ANovelmRNALevelSubtractionMethodforQuick
IdentificationofTarget-OrientatedUniquelyExpressed
GenesBetweenPeanutImmaturePodandLeaf
NingWu,KanyandMatand,andSonyaWilliams
Abstract
Subtractiontechniquehasbeenbroadlyappliedfortargetgenediscovery.However,mostcurrentproto-
colsapplyrelativedifferentialsubtractionandresultingreatamountclonemixturesofuniqueanddiffer-
entially expressed genes. This makesitmoredifficult toidentify unique ortarget-orientated expressed
genes.Inthisstudy,wedevelopedanovelmethodforsubtractionatmRNAlevelbyintegratingmagnetic
particletechnologyintodriverpreparationandtester–d
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