A quantitative PCR method for measuring absolute telomere length.docVIP

O’CallaghanandFenechBiologicalProceduresOnline2011,13:3 /content/13/1/3 Biological Procedures Online METHODOLOGY OpenAccess AquantitativePCRmethodformeasuring absolutetelomerelength NathanJO’Callaghan*,MichaelFenech Abstract Wedescribeasimpleandreproduciblemethodtomeasureabsolutetelomerelength(aTL)usingquantitativereal- timepolymerasechainreaction(qPCR).ThismethodisbasedontheCawthonmethodforrelativemeasurementof telomerelength(TL)butmodifiedbyintroducinganoligomerstandardtomeasureaTL.Themethoddescribesthe oligomerstandards,thegenerationofthestandardcurveandthecalculationsrequiredtocalculatea