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A scaling normalization method for differential expression analysis of RNA-seq data
RobinsonandOshlackGenomeBiology2010,11:R25
/2010/11/3/R25
METHOD
OpenAccess
Ascalingnormalizationmethodfordifferential
expressionanalysisofRNA-seqdata
MarkDRobinson1,2*,AliciaOshlack1*
Abstract
Thefinedetailprovidedbysequencing-basedtranscriptomesurveyssuggeststhatRNA-seqislikelytobecomethe
platformofchoiceforinterrogatingsteadystateRNA.Inordertodiscoverbiologicallyimportantchangesin
expression,weshowthatnormalizationcontinuestobeanessentialstepintheanalysis.Weoutlineasimpleand
effectivemethodforperformingnormalizationandshowdramaticallyimprovedresultsforinferringdifferential
expressioninsim
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