A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis.docVIP

BMC Plant Biology BioMedCentral Methodology article Open Access A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis Hirotaka Takahashi1,2, Akira Nozawa1,2,5, Motoaki Seki3, Kazuo Shinozaki4, Yaeta Endo*1,2,5 and Tatsuya Sawasaki*1,2,5 Address: 1Cell-Free Science and Technology Research Center, Ehime University, Matsuyama 790-8577, Japan, 2The Venture Business laboratory, Ehime University, Matsuyama 790-8577, Japan, 3Plant Functional Genomics Research Group, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa